FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241288
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG32581.3502536388050794Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG28831.1948377043201486Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT15200.6299525877789198No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT15140.6274659328271609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCAC14050.582291701203541No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11400.47246444083418987Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10770.4463545638407214Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCA10540.4368223865256457No Hit
GTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAA10460.43350684658996713No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9730.4032525446769007Clontech Universal Primer Mix Long (96% over 26bp)
CGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTG9680.4011803322171016No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9410.38999038493418653Clontech Universal Primer Mix Long (96% over 26bp)
CTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGTCT9380.38874705745830707No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA9360.3879181724743874Clontech Universal Primer Mix Long (96% over 26bp)
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA9300.38543151752262855No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGCAGCTC9130.3783859951593117No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT8600.35642054308544147No Hit
CACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAG8300.343987268326647No Hit
GTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCG7690.31870627631709825No Hit
GTTGTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGG7600.3149762938894599No Hit
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT7600.3149762938894599No Hit
ATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCC7540.312489638937701No Hit
TGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATG6910.28637976194423265No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6840.2834786645005139Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.2751898146613176No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC6450.2673154073140811Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG6340.2627565399025231Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6320.2619276549186035Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6290.2606843274427241Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6280.26026988495076425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTT6270.25985544245880443No Hit
GGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTC6170.2557110175392062No Hit
CCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAG6160.25529657504724645No Hit
GTACAGGCTATTGAGAATTTGCGTGTCCCTTTCATAGATTTTCAAGGTCCAAGGAGAGGGTACCGACACAGCAAT6090.25239547760352776No Hit
CTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCGCAGCAGGTCTC5910.24493551274825104No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5800.24037664533669306Clontech SMART CDS Primer II A (96% over 26bp)
ATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCG5720.23706110540101458No Hit
CTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTA5660.23457445044925565No Hit
GTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCACCATTACAGACTTAAT5580.23125891051357714No Hit
TAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCAC4990.206806803487948No Hit
GTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGGAAG4970.20597791850402838No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4830.20017572361659097Clontech Universal Primer Mix Long (96% over 26bp)
AAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAAC4690.19437352872915353No Hit
TTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC4450.18442690892211797No Hit
GTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCG4440.18401246643015814No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAG4410.1827691389542787No Hit
TTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGAC4410.1827691389542787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.17986804151056002No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAA4190.17365140413116276No Hit
GCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAG4170.17282251914724314No Hit
GATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAG4120.17075030668744404No Hit
CCTTTTAGGCACGCACGCCTTGCGACACACGCACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCAT4120.17075030668744404No Hit
GTCCAAGGAGAGGGTACCGACACAGCAATTTAATGCCATGCTCTACCAATCTGTTATACCATATCTCTCTATGAA4080.16909253671960478No Hit
GTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCCATTGGCTTCTCTATGAGAGCTTCTCGTT4010.1661914392758861No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3950.1637047843241272Clontech Universal Primer Mix Long (96% over 26bp)
GTCTAACAAAGAGCGAAAGGGAATACGGTTCCAATTCCGTAACCTATTGAGTATACGTTTGTCATTAGTCTCCAT3950.1637047843241272No Hit
CTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGCGTGTCCCTTTCATAGATTTTCA3890.1612181293723683No Hit
CCTTGCGACACACGCACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCT3850.15956035940452903No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTA3830.1587314744206094No Hit
CTGTTATACCATATCTCTCTATGAAAGACTTCCATGGTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGA3640.1508570670733729No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC3530.14629819966181493Clontech Universal Primer Mix Long (96% over 26bp)
GATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACAC3510.14546931467789528No Hit
ACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTG3510.14546931467789528No Hit
GACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCT3350.13883823480653823No Hit
GTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCAC3330.13800934982261862No Hit
AGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATT3310.13718046483869897No Hit
CTCAATAGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAG3280.13593713736281954No Hit
GTTATACCATATCTCTCTATGAAAGACTTCCATGGTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGATA3260.13510825237889992No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3230.13386492490302046Clontech Universal Primer Mix Long (96% over 26bp)
CTCTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGT3170.13137826995126156No Hit
AATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCC3150.13054938496734192No Hit
GTATAACAGCTGTAATTTCTTAGATCATATTCCCAGTACTTGGATAACTGTGGAAAATCTAGAGCTAATACATGC3030.12557607506382415No Hit
GCTCTGAAGACTGTGGCAGCTCGGGCTTTACAATATGCATTCGCTTCACGGTGAATGTATATTAAGTCTGTAATG3030.12557607506382415No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2980.12350386260402506Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGG2970.12308942011206524No Hit
ATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACT2950.12226053512814562No Hit
GTCTGTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCAGAACTGGCA2930.121431650144226No Hit
ACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGC2860.11853055270050729No Hit
CCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACC2840.11770166771658765No Hit
GTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCAGAACTGGCACGGT2840.11770166771658765No Hit
CTCCATGTGTGATCGCAAGGTTGTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGA2820.116872782732668No Hit
ATCGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTG2810.1164583402407082No Hit
CCCTTACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTG2810.1164583402407082No Hit
GTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGCGTGTC2750.1139716852889493No Hit
GAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAA2720.11272835781306986No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2700.11189947282915023Clontech Universal Primer Mix Long (96% over 26bp)
GTATACGTTTGTCATTAGTCTCCATGTGTGATCGCAAGGTTGTGTATGGGTTAAAGGTTAATGACATCTCGGTAA2670.11065614535327077No Hit
GTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCA2660.11024170286131095No Hit
GTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCT2660.11024170286131095No Hit
GAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACTAGAGA2640.10941281787739134No Hit
GGGTACCGACACAGCAATTTAATGCCATGCTCTACCAATCTGTTATACCATATCTCTCTATGAAAGACTTCCATG2640.10941281787739134No Hit
TCCCTTACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATT2610.10816949040151189No Hit
GGGTAGGATTACCCGCTAGACTTAAGCATATTAATCAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAAC2610.10816949040151189No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2550.105682835449753Clontech Universal Primer Mix Long (96% over 26bp)
CCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGA2500.10361062298995391No Hit
GTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGA2500.10361062298995391No Hit
ACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGCGTGTCCCTTTCATA2500.10361062298995391No Hit
GTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGAC2490.1031961804979941No Hit
GTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAA2490.1031961804979941No Hit
GCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGA2480.1027817380060343No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGC2480.1027817380060343No Hit
CGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACT2470.10236729551407447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.10195285302211465No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG2450.10153841053015483Clontech Universal Primer Mix Long (96% over 26bp)
GTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCACCATTACAGACTTAATATACATTCACCGTG2430.10070952554623519No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT2420.1002950830542754Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)