FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241288
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG25881.0725771691920027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20420.8462915685819435Clontech Universal Primer Mix Long (96% over 26bp)
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCAC12770.5292430622326846No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11800.4890421405125825Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAA10060.41692914691157457No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCA9620.39869367726534266No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9110.37755711017539206Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGCAGCTC9000.3729982427638341No Hit
CGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTG8840.366367162892477No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG8660.3589071980372004Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT8430.3493750207221246No Hit
CTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGTCT8300.343987268326647No Hit
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA8270.34274394085076754No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8080.33486953350353105Clontech Universal Primer Mix Long (96% over 26bp)
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT7780.3224362587447366No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT7650.31704850634925896No Hit
GTTGTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGG7290.3021285766387056No Hit
ATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCC7280.3017141341467458No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7160.296740824243228Clontech Universal Primer Mix Long (96% over 26bp)
CACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAG7090.29383972679950926No Hit
TGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATG7030.2913530718477504No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6750.27974868207287557Clontech Universal Primer Mix Long (96% over 26bp)
GTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCG6690.2772620271211167No Hit
CCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAG6120.25363880507940717No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG6060.2511521501276483Clontech Universal Primer Mix Long (96% over 25bp)
CTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCGCAGCAGGTCTC5760.23871887536885383No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5690.23581777792513509Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGGCTATTGAGAATTTGCGTGTCCCTTTCATAGATTTTCAAGGTCCAAGGAGAGGGTACCGACACAGCAAT5610.23250223798945657No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5410.22421338815026026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCACCATTACAGACTTAAT5380.22297006067438083No Hit
GGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTC5350.2217267331985014No Hit
CTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTA5330.22089784821458175No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5210.21592453831106398Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5040.20887901594774708Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5020.20805013096382743Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGGAAG4490.18608467888995725No Hit
TTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGAC4420.1831835814462385No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4420.1831835814462385Clontech SMART CDS Primer II A (96% over 26bp)
CTGTTATACCATATCTCTCTATGAAAGACTTCCATGGTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGA4420.1831835814462385No Hit
TAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCAC4260.17655250157488148No Hit
GATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAG4120.17075030668744404No Hit
TTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC4040.16743476675176552No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3930.16287589934020755Clontech Universal Primer Mix Long (96% over 26bp)
ATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCG3850.15956035940452903No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTT3800.15748814694472996No Hit
GTCCAAGGAGAGGGTACCGACACAGCAATTTAATGCCATGCTCTACCAATCTGTTATACCATATCTCTCTATGAA3750.15541593448493088No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAA3720.15417260700905144No Hit
GCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAG3690.15292927953317198No Hit
GTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCCATTGGCTTCTCTATGAGAGCTTCTCGTT3630.15044262458141308No Hit
CTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGCGTGTCCCTTTCATAGATTTTCA3620.15002818208945326No Hit
GATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACAC3580.148370412121614No Hit
CCTTTTAGGCACGCACGCCTTGCGACACACGCACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCAT3560.1475415271376944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.14546931467789528No Hit
GTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCAC3480.14422598720201585No Hit
CCTTGCGACACACGCACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCT3430.14215377474221677No Hit
GTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCG3370.13966711979045787No Hit
GCTCTGAAGACTGTGGCAGCTCGGGCTTTACAATATGCATTCGCTTCACGGTGAATGTATATTAAGTCTGTAATG3260.13510825237889992No Hit
GTCTAACAAAGAGCGAAAGGGAATACGGTTCCAATTCCGTAACCTATTGAGTATACGTTTGTCATTAGTCTCCAT3220.13345048241106064No Hit
CTCAATAGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAG3210.13303603991910082No Hit
GTTATACCATATCTCTCTATGAAAGACTTCCATGGTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGATA3160.13096382745930174No Hit
GTCTGTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCAGAACTGGCA3130.1297204999834223No Hit
GACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCT3120.12930605749146248No Hit
AAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAAC3080.12764828752362323No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3070.1272338450316634Clontech Universal Primer Mix Long (96% over 26bp)
AGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATT3000.1243327475879447No Hit
CTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGG3000.1243327475879447No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2990.12391830509598488Clontech Universal Primer Mix Long (96% over 26bp)
GTATAACAGCTGTAATTTCTTAGATCATATTCCCAGTACTTGGATAACTGTGGAAAATCTAGAGCTAATACATGC2970.12308942011206524No Hit
AATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCC2960.12267497762010543No Hit
CTCTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGT2950.12226053512814562No Hit
ACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGC2930.121431650144226No Hit
CCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACC2880.11935943768442692No Hit
ACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTG2850.11811611020854747No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGC2810.1164583402407082No Hit
ATCGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTG2770.11480057027286894No Hit
GTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGCGTGTC2700.11189947282915023No Hit
GTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCA2680.11107058784523059No Hit
CGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACT2670.11065614535327077No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAG2610.10816949040151189No Hit
CTCCATGTGTGATCGCAAGGTTGTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGA2600.10775504790955207No Hit
GTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCAGAACTGGCACGGT2590.10734060541759224No Hit
ATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACT2580.10692616292563242No Hit
GTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGAC2530.10485395046583336No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTA2490.1031961804979941No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2490.1031961804979941Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAGGATTACCCGCTAGACTTAAGCATATTAATCAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAAC2490.1031961804979941No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT2470.10236729551407447Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)