FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159948
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.5633080751244154No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG8780.5489284017305625Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG7240.45264711031085103Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAG7060.4413934528721835No Hit
GTGGTACTAAGCGTACGACAAGTAGGGCGGGACACGAGAAATCCTGTCTGAACATGGGGGGACCATCCTCCAAGG6370.39825443269062444No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCAGTTTATTGCAAC6180.3863755720609198No Hit
GTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCCAGCTGTTGGAATAGCTGGTTTCTGGTTCATAGA5920.37012028909395556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.3451121614524721No Hit
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCA5340.3338585040138045No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGAAAAGGTACGCC5310.33198289444069323No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCCAGCTGTTGGAATAGCTGGTTTCT5100.3188536274289144No Hit
GGTCTACACCCAGCGACTGTCGCCCTGTTCGGACTCGATTTCTCTACGGCTCCCCTATCCGGTTAACCTCGCCAC4950.3094755795633581No Hit
GTTATGGCTAGGGGGTCATTGCGACTTACCAAACCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGAGA4790.2994723285067647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.2982219221246905No Hit
AGCCATAACAGTGCTCTACCCCCGATGGTGATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTAT4740.2963463125515793No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT4370.27321379448320704No Hit
ATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATT4360.27258859129216995No Hit
GTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT4170.2607097306624653No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCAGTTTATTGCAACCT4140.25883412108935405No Hit
ATACGAGACCGTACCGCAAACCGACACAGGTGGGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCGGGAGAA4140.25883412108935405No Hit
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCTCCG4000.2500812764148348No Hit
CCTTAGAATACTCATCTCGCCCACCTGTGTCGGTTTGCGGTACGGTCTCGTATGACTGAAGCTTAGAGGCTTTTC3960.24758046365068648No Hit
GTGCTGTGTTTTTATTAAACAGTCGCAGCCACCAGTTTATTGCAACCTTTTCGCCCTTCCACAGTAAAGTGGTCA3930.24570485407757522No Hit
GGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCGTCGTA3870.2419536349313527No Hit
GTGTAGACCCGAAACCAAGTGATCTACCCATGGCCAGGATGAAGGTGCGGTAACACGCCCTGGAGGTCCGAACCC3830.23945282216720434No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA3820.23882761897616725No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3810.23820241578513018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCT3580.2238227423912772No Hit
TAGTACCACCGTCCGGATTTCGTGTACGGGGCTATCACCCGCTATGGCTGCCATTTCCAGAGCATTCCACTATCC3520.22007152324505463No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGG3290.20569184985120165No Hit
GTGCGAGCTTGGGAGACAGACGTCGGGTGCTAACGTCCGGCGTCAAGAGGGAAACAACCCAGACCGCCAGCTAAG3230.2019406307049791No Hit
CCGTAAGCCCAAGGTTTCCTGTTCAACGTTCATCGGAGCAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCG3170.1981894115587566No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGC3120.19506339560357117No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTT3060.19131217645734863No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2980.18631055092905194Clontech Universal Primer Mix Long (96% over 26bp)
TGGTAGGAGAGCGTTCTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTG2950.18443494135594068No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCG2750.17193087753519892No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGTGTGCATACA2730.17068047115312476No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGCGGCCGTAACAATAACGGT2690.16817965838897642No Hit
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGTGAGCAAACACAC2660.16630404881586514No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTA2510.15692600095030884No Hit
GAGCAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCG2510.15692600095030884No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2480.1550503913771976Clontech Universal Primer Mix Long (96% over 26bp)
GACTCACCCTGCTCCGATGAACGTTGAACAGGAAACCTTGGGCTTACGGCGTGCGGGCTTTTCACCCGCATTATC2470.1544251881861605No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG2380.1487983594668267Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.1444219371295671No Hit
GGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATTCCCAAAG2280.14254632755645585No Hit
TCCCAGTCCCTTGAATTACGCACCCGGATTTGCCTAAGTGCCTTCTATGAACCAGAAACCAGCTATTCCAACAGC2260.14129592117438167No Hit
CTTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA2230.1394203116012704No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2210.13816990521919625Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCGTATGACTGAAGCTTAGAGGCTTTTCTTGGAACCACTTCCGATTGCTTCGTGGCGCATGGCCACACGTCC2150.13441868607297372No Hit
GGACTGGGACGTGTGGCCATGCGCCACGAAGCAATCGGAAGTGGTTCCAAGAAAAGCCTCTAAGCTTCAGTCATA2080.13004226373571412No Hit
CTCCTACCATATGTCAAAGACATATCCGCAGCTTCGGTGACTGGCTTAGCCCCGTTACATCTTCCGCGCAGGACG2010.1256658413984545No Hit
CTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAGCGATA1990.12441543501638033No Hit
ACGTAGTAGCTTGCGATAAGCTGCGGGGAGTGAGCAAACACACTTTGATCCGCAGATTTCCGAATGGGGAAACCC1970.12316502863430615No Hit
TCGGTAGCTTGACCACTTTACTGTGGAAGGGCGAAAAGGTTGCAATAAACTGGTGGCTGCGACTGTTTAATAAAA1960.12253982544326905No Hit
AGCTCGCACTCATCGGTATTCGGAGTTTGCAATGGTTTGGTAAGTCGCAATGACCCCCTAGCCATAACAGTGCTC1940.12128941906119489No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC1890.11816340310600945Clontech Universal Primer Mix Long (96% over 26bp)
GGCTTACGGCGTGCGGGCTTTTCACCCGCATTATCGCTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA1880.11753819991497237No Hit
AACCGACACAGGTGGGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGT1850.1156625903418611No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1810.11316177757771276Clontech Universal Primer Mix Long (96% over 26bp)
GACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAG1800.11253657438667568No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC1790.1119113711956386No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1760.11003576162252732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTCGGGA1750.10941055843149024No Hit
GTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCAT1740.10878535524045316No Hit
CACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCAGTTTATTGCA1720.10753494885837897No Hit
AGCTACCGAGGCGTACCTTTTCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCG1700.10628454247630481No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1660.10378372971215645Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCAGTTTAT1660.10378372971215645No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCT1650.10315852652111937No Hit
GTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCG1650.10315852652111937No Hit
TAGTACCACCGTCCGGATTTCGTGTACGGGGCTATCACCCGCTATGGCCAGAGTTTCCAATCTGTTCCACTATCC1620.1012829169480081No Hit
GTTCGGACTCGATTTCTCTACGGCTCCCCTATCCGGTTAACCTCGCCACTGAATGTAAGTCGCTGACCCATTATA1620.1012829169480081No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGG1620.1012829169480081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)