FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233197
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGG12060.5171593116549527No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10110.43353902494457475Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGA8190.35120520418358725No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG7700.33019292701021025No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG7690.3297641050270801Clontech Universal Primer Mix Long (96% over 26bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGG7640.3276199951114294No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT6830.2928854144778878No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCG6480.27787664506833276No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAACCAGTTAAAAT6410.2748748911864218No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAG6210.2662984515238189No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT6090.2611525877262572No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCA6080.26072376574312706No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC5250.22513154114332518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.2122668816494209No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA4930.2114092376831606No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGC4910.2105515937169003No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTC4860.20840748380124957No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAATGTGTAACAACTCACCTGCCGAATGA4810.2062633738855989No Hit
GAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA4620.19811575620612615No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC4600.19725811223986586No Hit
ACTTATACCCCCCCGCCGGGGCAAAATTTACGCCCCGGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTG4580.19640046827360558No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGG4520.19382753637482472No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4430.18996813852665342Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTATTCAGTTTTATGAGGTAAAGCGAAT4400.188681672577263No Hit
GCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCA4380.18782402861100272No Hit
GTTGGACACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGA4340.18610874067848215No Hit
CCCCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC4260.182678164813441No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT4000.17152879325205728No Hit
GCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC3990.17109997126892712No Hit
GGATATGGATTCTCCGCGGCAACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCT3960.1698135053195367No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3880.16638292945449557No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAG3710.15909295574128313No Hit
CCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCCGGGGCGTAAATTTTGCCCCGGCGGGGGGGTAT3670.15737766780876256No Hit
TCCATATCCAGATACCAGAATTTTAACTGGTTTCCCTTTCGAGGGCGCGGCGCAAGCGCCGACATTAAAACGGAA3600.15437591392685154No Hit
GTCTAGATGAACCAACACCTTTTGTGGTGTCTGATGAGCGTGTATTCCGGCACTTTAACCCCCCGTTCGGTTCAT3570.15308944797746113No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT3510.15051651607868027No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCCGGGGCGTAAATTTTGCCCCGG3510.15051651607868027No Hit
GTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTAC3440.14751476219676926No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCC3430.1470859402136391No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT3420.14665711823050898No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGT3400.1457994742642487No Hit
CTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTT3390.14537065228111853No Hit
CATCAGACACCACAAAAGGTGTTGGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA3370.14451300831485825No Hit
ATCCTGATGTCTTCGGAAGGATTTGAGTAAGAGCATGGCTGTTGGGACCCGAAAGATGGTGAACTATGCTTGAAT3360.14408418633172812No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA3340.14322654236546783No Hit
GGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAACCAGTTAAAATTCTGGTATCTGGA3330.14279772038233768No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG3300.14151125443294726No Hit
CTACTAGATAGTTCGATTAGTCTTTCGCCCCTATGCCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG3250.13936714451729654No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3240.13893832253416638Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC3200.1372230346016458No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3190.13679421261851568Clontech Universal Primer Mix Long (96% over 26bp)
GTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCC3150.1350789246859951No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT3120.13379245873660467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.13293481477034438No Hit
ACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA3080.1320771708040841No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACT3030.12993306088843337No Hit
CGTCGGGAGCGCACCAAACGCCGCAAAAGTGCAGCGCTCTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGAT3000.12864659493904296No Hit
GCACTAGAGGCCGTTCGACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCCGGGG2970.12736012898965252No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTG2950.12650248502339223No Hit
GTGCCCTTCTGCGCCAGAGGCGTCTCCCCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT2940.12607366304026207No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC2910.12478719709087167Clontech Universal Primer Mix Long (96% over 26bp)
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG2890.12392955312461137No Hit
GCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCTCCCCAAGGCCCAACGCACCCAA2890.12392955312461137No Hit
GTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATC2890.12392955312461137No Hit
TGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCT2890.12392955312461137No Hit
CCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCTCCCCAAGGCCCAACGCA2890.12392955312461137No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTATACCCCCCCG2850.12221426519209082No Hit
CATCAGGATCGGTCGATAGTGCACCCTTGCGGGTTCCTACCTCCGTTCACTTTCATTACGCGCTCGGGCTTGACA2840.12178544320896068No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG2840.12178544320896068No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2830.12135662122583052Clontech Universal Primer Mix Long (96% over 26bp)
CTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCATATCCAGATACCAGAATT2820.12092779924270038No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGAC2770.11878368932704966No Hit
GTTGAATGAAACGTTATTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGGGGGGTT2740.11749722337765924No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA2730.11706840139452908No Hit
CTCTAAGGGTTGGGTGCGTTGGGCCTTGGGGAGACGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCC2710.1162107574282688No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2680.11492429147887838Clontech Universal Primer Mix Long (96% over 26bp)
GGTATAAGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACATTCCT2670.11449546949574824No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGC2650.11363782552948794No Hit
GCCTTATAGGGGAGACGACATATTACCAGCCTGGACTGAGGTCCGCGCATCTGCTAGGATGCTGGCGTAATGGCT2630.11278018156322767No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGC2620.11235135958009752No Hit
CTCGAAAGGGAAACCAGTTAAAATTCTGGTATCTGGATATGGATTCTCCGCGGCAACGCAACTGAGAGCGGAGAC2570.1102072496644468No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGGGGAGACGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGC2540.10892078371505637No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAA2540.10892078371505637No Hit
CCACAAAAGGTGTTGGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAATGTGTAAC2530.10849196173192623No Hit
GTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGC2520.10806313974879608No Hit
GGATAGCAGTAACGTATTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTGGGGGCTGAAACAGCCTTCACC2490.10677667379940564No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA2470.10591902983314537No Hit
GTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTG2460.10549020785001523No Hit
CTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAA2450.10506138586688508No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2390.10248845396810423Clontech Universal Primer Mix Long (96% over 25bp)
CTCTTAGGATCGATTGACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTC2380.10205963198497409No Hit
TCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTG2380.10205963198497409No Hit
ATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC2380.10205963198497409No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGG2370.10163081000184393No Hit
CTAGAGGCCGTTCGACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCCGGGGCGT2370.10163081000184393No Hit
GGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAACC2360.10120198801871379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)