FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325474
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGATGAAATATACCACCCATTTTGAGCTGCATT19070.5859146967192465No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.5856074525154082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12280.3772958823131802No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG10150.31185286689566605No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT9330.2866588421809423No Hit
TTCCCAAACAACTCGACTCGTCGAAGGGGTTTCACATGGAGTCGGTCACCGGCCGCATACGGGATTCTCACCCTC9180.2820501791233708No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACG9140.28082120230801844No Hit
GTATTTAGCTTTAGATGAAATATACCACCCATTTTGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGGGTTT8370.25716339861248516No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGCCGGCGCCTCGCCCGAAACACCCTCTACAAATTACAACTCGGAC8350.25654891020480897No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCAGGTGCTGGAAGG8310.2553199333894566No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA6380.19602180204870437No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG6210.19079865058345674No Hit
GAAATATACCACCCATTTTGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGGGTTTCACATGGAGTCGGTCA6140.1886479411565901No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACA5980.18373203389518056No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCAGG5980.18373203389518056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.1831175454875044No Hit
GTTCTAAGTTGGTTGTTAATTGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCAGAACCCCGATGCTGCTCGG5910.1815813244683139No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT5900.1812740802644758No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA5880.1806595918567996No Hit
TTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTG5840.17943061504144725No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC5680.17451470778003772No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAA5390.16560462586873298No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.16191769542267587No Hit
CTATGACGTCCTGTTCCAAGGAACTTAGGCCGGCGCCTCGCCCGAAACACCCTCTACAAATTACAACTCGGACTC5010.1539293461228854No Hit
TCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTG4950.1520858808998568No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT4910.15085690408450445No Hit
GTGCTGGAAGGCGCCGGCCCGTAAAGGCTAGTGCCGACCTGCTCCCGGTGCTTCCACCCAAGGCCCAACGTACCC4850.1490134388614759No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA4800.1474772178422854No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCG4790.1471699736384473No Hit
CCTCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTTCTTCGGAGTCCGAGTTGTAATTTG4780.1468627294346092No Hit
GTCATAGAGGGTGAGAATCCCGTATGCGGCCGGTGACCGACTCCATGTGAAACCCCTTCGACGAGTCGAGTTGTT4650.142868554784714No Hit
TGCCAAGGGCGTCCACAACCAGGTGCTGGAAGGCGCCGGCCCGTAAAGGCTAGTGCCGACCTGCTCCCGGTGCTT4510.13856713593098066No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC4330.13303674026189496No Hit
GTATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGC4200.12904256561199973No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC4090.1256628793697807No Hit
AGCGGCAACAGCTCAAATTTGAAATCTGGCTTCTTCGGAGTCCGAGTTGTAATTTGTAGAGGGTGTTTCGGGCGA4030.12381941414675213No Hit
ATTTTGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGGGTTTCACATGGAGTCGGTCACCGGCCGCATACGG3960.12166870471988546No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGG3950.12136146051604736No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTG3910.12013248370069497No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAAC3880.11921075108918071No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCAGAA3850.11828901847766643No Hit
GGTCGGCACTAGCCTTTACGGGCCGGCGCCTTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCC3840.11798177427382833No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3780.11613830905079976No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC3740.11490933223544737No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA3720.11429484382777118No Hit
GCCCGGCACTAGCTTGGCAACAGGCCGGCGCCGAGCAGCATCGGGGTTCTGACGCCCTTGGCAGGCTTCGGCCGT3690.1133731112162569No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGTGGAAGCACCGGGAGCAGGTCGGCACTAGCCTTTACGGGCCGGC3680.1130658670124188No Hit
CAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTTCTTCGGAGTCCGAGTTGTAATTTGTAG3660.11245137860474264No Hit
CTTGTGCGTCGCCCGGCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTGGATTCTCCACGGCAACGTAA3650.11214413440090454No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT3630.11152964599322834No Hit
ATGACGTCCTGTTCCAAGGAACTTAGGCCGGCGCCTCGCCCGAAACACCCTCTACAAATTACAACTCGGACTCCG3570.10968618077019977No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATTGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCAGAACCCCGA3550.10907169236252358No Hit
ACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAACA3530.10845720395484738No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT3490.10722822713949501No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT3460.10630649452798074No Hit
GCCGAAGCCTGCCAAGGGCGTCCACAACCAGGTGCTGGAAGGCGCCGGCCCGTAAAGGCTAGTGCCGACCTGCTC3420.10507751771262835No Hit
AGCCTTTACGGGCCGGCGCCTTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCT3400.10446302930495216No Hit
GCACTAGCCTTTACGGGCCGGCGCCTTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGT3380.10384854089727598No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGTGGAAGCACCGGGAGCAGGTCGGCACTAGCCTTTACGGGCCGGCG3370.10354129669343788No Hit
GAGTCGGTCACCGGCCGCATACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGGCCGGCGCCTC3370.10354129669343788No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)