FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325474
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGATGAAATATACCACCCATTTTGAGCTGCATT17500.5376773567166655No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG9670.2971051451114375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.2869660863847804No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT8610.26453725950459944No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACG8600.2642300153007614No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCAGGTGCTGGAAGG8450.25962135224318994No Hit
GTATTTAGCTTTAGATGAAATATACCACCCATTTTGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGGGTTT7680.23596354854765667No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGCCGGCGCCTCGCCCGAAACACCCTCTACAAATTACAACTCGGAC7650.23504181593614237No Hit
TTCCCAAACAACTCGACTCGTCGAAGGGGTTTCACATGGAGTCGGTCACCGGCCGCATACGGGATTCTCACCCTC7650.23504181593614237No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA6580.20216668612546623No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG6060.1861899875258853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.1828103012836663No Hit
GTTCTAAGTTGGTTGTTAATTGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCAGAACCCCGATGCTGCTCGG5880.1806595918567996No Hit
TTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTG5630.17297848676084726No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC5520.16959880051862822No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA5370.16499013746105679No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAA5310.16314667223802823No Hit
GTCATAGAGGGTGAGAATCCCGTATGCGGCCGGTGACCGACTCCATGTGAAACCCCTTCGACGAGTCGAGTTGTT5230.16068871860732348No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACA5160.1585380091804568No Hit
GAAATATACCACCCATTTTGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGGGTTTCACATGGAGTCGGTCA5160.1585380091804568No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCAGG5150.1582307649766187No Hit
CTATGACGTCCTGTTCCAAGGAACTTAGGCCGGCGCCTCGCCCGAAACACCCTCTACAAATTACAACTCGGACTC5050.15515832293823778No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT4990.1533148577152092No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA4830.1483989504537997No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT4800.1474772178422854No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCG4410.13549469389259972No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC4260.1308860308350283No Hit
GTGCTGGAAGGCGCCGGCCCGTAAAGGCTAGTGCCGACCTGCTCCCGGTGCTTCCACCCAAGGCCCAACGTACCC4190.12873532140816163No Hit
CCTCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTTCTTCGGAGTCCGAGTTGTAATTTG4150.12750634459280924No Hit
TCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTG4070.12504839096210452No Hit
TGCCAAGGGCGTCCACAACCAGGTGCTGGAAGGCGCCGGCCCGTAAAGGCTAGTGCCGACCTGCTCCCGGTGCTT4070.12504839096210452No Hit
AGCGGCAACAGCTCAAATTTGAAATCTGGCTTCTTCGGAGTCCGAGTTGTAATTTGTAGAGGGTGTTTCGGGCGA4060.12474114675826642No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGTGGAAGCACCGGGAGCAGGTCGGCACTAGCCTTTACGGGCCGGC4000.12289768153523783No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC3950.12136146051604736No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA3850.11828901847766643No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGG3770.11583106484696166No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3750.11521657643928547No Hit
GCCCGGCACTAGCTTGGCAACAGGCCGGCGCCGAGCAGCATCGGGGTTCTGACGCCCTTGGCAGGCTTCGGCCGT3710.1139875996239331No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTG3710.1139875996239331No Hit
ATGACGTCCTGTTCCAAGGAACTTAGGCCGGCGCCTCGCCCGAAACACCCTCTACAAATTACAACTCGGACTCCG3710.1139875996239331No Hit
GGTCGGCACTAGCCTTTACGGGCCGGCGCCTTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCC3670.11275862280858073No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAAC3590.11030066917787597No Hit
CAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTTCTTCGGAGTCCGAGTTGTAATTTGTAG3570.10968618077019977No Hit
AGCCTTTACGGGCCGGCGCCTTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCT3500.1075354713433331No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATTGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCAGAACCCCGA3480.10692098293565691No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT3470.10661373873181881No Hit
GTATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGC3360.10323405248959978No Hit
ATTTTGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGGGTTTCACATGGAGTCGGTCACCGGCCGCATACGG3360.10323405248959978No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCAGAA3350.10292680828576169No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGTGGAAGCACCGGGAGCAGGTCGGCACTAGCCTTTACGGGCCGGCG3340.10261956408192359No Hit
ACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAACA3310.10169783147040931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.10169783147040931No Hit
CTTGTGCGTCGCCCGGCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTGGATTCTCCACGGCAACGTAA3310.10169783147040931No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)