FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246639
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG22780.9236171084054022Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG22020.8928028413997787Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13310.5396551234800661Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.39855821666484215No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9810.3977473149015362Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG9290.37666386905558324Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8170.33125337031045377Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC8010.3247661562040067Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7820.3170625894526008Clontech Universal Primer Mix Long (96% over 26bp)
GTCAAGGACGCACCGGACACAACACAAGTGCTGTGCTCTACCCGCCATATAACCCTAGCTCCGGATAATCCGATT7290.29557369272499484No Hit
ACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGG7210.2923300856717713No Hit
GTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGTACCCAACCCTT6640.2692193854175536No Hit
GTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAAGTCTCACGCCTGGTCGTACTCATAACCGCAGCAGGTC6560.26597577836433006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.26029946602118886No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC6290.2550286045597006Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6110.24773048868994765Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5740.23272880606878882Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGAAGGCTGCTAAAAGAGTCCTCTCGCAGGTCCGAACCAG5590.22664704284399467No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5530.22421433755407702Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5440.22056527961920053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT5230.21205081110448873No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTTATAAGTTCCGTCGG4690.19015646349522988No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGA4660.18894011085027104No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTCG4640.18812920908696515No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG4270.17312752646580631Clontech Universal Primer Mix Long (96% over 25bp)
ATCCTAAGAGATAGGGAAACTCCGTTTTAAAGCGTTTGATTTTTCAAGCCGCCTATCGAAAGGGAATCCGGTTAA4270.17312752646580631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.16826211588597098No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4070.16501850883274746Clontech Universal Primer Mix Long (96% over 26bp)
GGTCCGAACCAGTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGT4000.16218035266117686No Hit
GGGTTATATGGCGGGTAGAGCACAGCACTTGTGTTGTGTCCGGTGCGTCCTTGACAGTCCTTGAAAATCCGACGG3960.16055854913456508No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA3770.15285498238315917No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3740.15163862973820036No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT3740.15163862973820036No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3730.1512331788565474No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3720.1508277279748945Clontech SMART CDS Primer II A (96% over 26bp)
CTCCCAGTCCGACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGTACCCAACCCTTAGAGCCAATCCTTTTCC3680.14920592444828273No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3600.14596231739505916Clontech Universal Primer Mix Long (96% over 26bp)
GCTAAAAGAGTCCTCTCGCAGGTCCGAACCAGTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCCGCTCGA3570.14474596475010035No Hit
GAGTACGACCAGGCGTGAGACTTATAAGTTCCGTCGGATTTTCAAGGACTGTCAAGGACGCACCGGACACAACAC3420.13866420152530623No Hit
GTCCGACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT3420.13866420152530623No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTTATAAG3420.13866420152530623No Hit
ACCCATACCTCACCGTCAGCGTTTGAGTGACGCGCTGACGAGTAGGCAGGCGTGGAGGTTTAGTGTAGAAGCTTT3240.13136608565555324No Hit
GTCCGTACCAGTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGAAGGCTGCTAAAAGAGTCCTCTCGCAGG3180.1289333803656356No Hit
GTTGTTAAGCGTACGCCAGACGCCCGAAGGCTGCTAAAAGAGTCCTCTCGCAGGTCCGAACCAGTCCAAGAAGGT3160.12812247860232973No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCG3150.12771702772067678No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCACCG3110.12609522419406502No Hit
CCCCTTGTCCGTACCAGTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGAAGGCTGCTAAAAGAGTCCTCT3110.12609522419406502No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3070.12447342066745325No Hit
GCACTTGTGTTGTGTCCGGTGCGTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAAGTCTCACGCCTGGTC3040.12325706802249442No Hit
ATATAACCCTAGCTCCGGATAATCCGATTTCAGGGTCGTAAGCGGTTAAGGAGAAAAGAGAACTCTTCCCAGGGC3030.12285161714084149No Hit
CTTGTGTTGTGTCCGGTGCGTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAAGTCTCACGCCTGGTCGTA2970.12041891185092382No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2970.12041891185092382Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTTAACCGCTTACGACCCTGAAATCGGATTATCCGGAGCTAGGGTTATATGGCGGGTAGAGCACAGCACTTG2940.119202559205965No Hit
CAACACAAGTGCTGTGCTCTACCCGCCATATAACCCTAGCTCCGGATAATCCGATTTCAGGGTCGTAAGCGGTTA2890.11717530479770027No Hit
TCCTTAACCTATTCTCAAACTTTAAATGTGTAAGACGATTTTGTTACTTAATTGAACGAAATCATGCGAATGAGA2860.11595895215274145No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGAAGGCTGCTAAAAG2850.11555350127108852No Hit
GGACTGGGTCGGACTGGGAGGGGCAACCTTCTTGGACTGGTTCGGACCTGCGAGAGGACTCTTTTAGCAGCCTTC2840.11514805038943558No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2740.11109354157290616Clontech Universal Primer Mix Long (96% over 26bp)
GGGTTAGTCGATCCTAAGAGATAGGGAAACTCCGTTTTAAAGCGTTTGATTTTTCAAGCCGCCTATCGAAAGGGA2720.11028263980960026No Hit
CCATATAACCCTAGCTCCGGATAATCCGATTTCAGGGTCGTAAGCGGTTAAGGAGAAAAGAGAACTCTTCCCAGG2720.11028263980960026No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2720.11028263980960026Clontech Universal Primer Mix Long (96% over 26bp)
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTTATAAGTTCCGTCGGATTTTCAAGGACTGT2670.10825538540133554No Hit
CCCGAAGGCTGCTAAAAGAGTCCTCTCGCAGGTCCGAACCAGTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAG2640.10703903275637672No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA2590.10501177834811203No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA2550.10338997482150025No Hit
GTCCGAACCAGTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGTA2520.10217362217654143No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT2500.10136272041323553No Hit
GTCCTTGAAAATCCGACGGAACTTATAAGTCTCACGCCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGA2480.10055181864992967No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA2480.10055181864992967No Hit
TTCAAGGACTGTCAAGGACGCACCGGACACAACACAAGTGCTGTGCTCTACCCGCCATATAACCCTAGCTCCGGA2470.1001463677682767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.1001463677682767No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2470.1001463677682767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)