FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246639
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG18710.7585985995726549Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG14560.5903364836866838Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9880.4005854710731068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.33125337031045377No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7480.3032772594764007Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6650.2696248362992065Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6550.2655703274826771Clontech Universal Primer Mix Long (96% over 26bp)
ACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGG6360.2578667607312712No Hit
GTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAAGTCTCACGCCTGGTCGTACTCATAACCGCAGCAGGTC6060.24570323428168298No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG5920.24002692193854175Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC5690.23070155166052408Clontech Universal Primer Mix Long (96% over 26bp)
GTCAAGGACGCACCGGACACAACACAAGTGCTGTGCTCTACCCGCCATATAACCCTAGCTCCGGATAATCCGATT5630.2282688463706064No Hit
GTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGTACCCAACCCTT5350.21691622168432406No Hit
GTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGAAGGCTGCTAAAAGAGTCCTCTCGCAGGTCCGAACCAG4930.1998872846549005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.19867093200994168No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4690.19015646349522988Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4440.18002019145390633Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4430.17961474057225338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4370.17718203528233573Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4260.17272207558415334Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT4220.17110027205754158No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTTATAAGTTCCGTCGG4070.16501850883274746No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTCG3840.1556931385547298No Hit
GCTAAAAGAGTCCTCTCGCAGGTCCGAACCAGTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCCGCTCGA3730.1512331788565474No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3670.14880047356662976Clontech Universal Primer Mix Long (96% over 25bp)
GGGTTATATGGCGGGTAGAGCACAGCACTTGTGTTGTGTCCGGTGCGTCCTTGACAGTCCTTGAAAATCCGACGG3660.14839502268497684No Hit
GGTCCGAACCAGTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGT3510.1423132594601827No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3490.14150235769687683No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGA3490.14150235769687683No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3390.1374478488803474No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA3350.1358260453537356No Hit
CTCCCAGTCCGACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGTACCCAACCCTTAGAGCCAATCCTTTTCC3320.1346096927087768No Hit
GTCCGTACCAGTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGAAGGCTGCTAAAAGAGTCCTCTCGCAGG3270.13258243830051208No Hit
CCCCTTGTCCGTACCAGTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGAAGGCTGCTAAAAGAGTCCTCT3270.13258243830051208No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT3210.13014973301059443No Hit
GTCCGACCCAGTCCCGCTCGATTCCTCTAATAGCCCGACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT3150.12771702772067678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.12447342066745325No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTTATAAG3060.12406796978580029No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3040.12325706802249442Clontech SMART CDS Primer II A (96% over 26bp)
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCG2800.1135262468628238No Hit
TCCTTAACCTATTCTCAAACTTTAAATGTGTAAGACGATTTTGTTACTTAATTGAACGAAATCATGCGAATGAGA2760.11190444333621204No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAAAGCGTTTGATTTTTCAAGCCGCCTATCGAAAGGGAATCCGGTTAA2760.11190444333621204No Hit
ACCCATACCTCACCGTCAGCGTTTGAGTGACGCGCTGACGAGTAGGCAGGCGTGGAGGTTTAGTGTAGAAGCTTT2750.11149899245455909No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2710.10987718892794733Clontech Universal Primer Mix Long (96% over 26bp)
CTTGTGTTGTGTCCGGTGCGTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAAGTCTCACGCCTGGTCGTA2570.10420087658480613No Hit
GCACTTGTGTTGTGTCCGGTGCGTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAAGTCTCACGCCTGGTC2570.10420087658480613No Hit
GTTGTTAAGCGTACGCCAGACGCCCGAAGGCTGCTAAAAGAGTCCTCTCGCAGGTCCGAACCAGTCCAAGAAGGT2570.10420087658480613No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA2470.1001463677682767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)