FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411151
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57281.393162122918344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35490.8631865178486736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.5946720304705571No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA16940.4120140775530158RNA PCR Primer, Index 33 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.3675048826343606No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.21816802099471969No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.17560458323097838No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.14495890804108466No Hit
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5630.13693265977706487RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4660.11334035427373397RNA PCR Primer, Index 20 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)