FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166762
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45392.7218431057435146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29251.753996713879661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20011.1999136493925473No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.803540374905554No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA9920.5948597402285893RNA PCR Primer, Index 33 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.5025125628140703No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.37838356460104816No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.33760688885957235No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.2920329571485111No Hit
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA3280.19668749475300126RNA PCR Primer, Index 33 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1950.11693311425864406No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.11213585828905866No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.10973723030426596No Hit
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.10913757330806778No Hit
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA1690.10134203235749152RNA PCR Primer, Index 20 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT1670.10014271836509517No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)