EGAF00002149536 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00002149536
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21952
Sequences flagged as poor quality	0
Sequence length	75
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	3813	17.369715743440235	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA	1006	4.582725947521866	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	846	3.853862973760933	RNA PCR Primer, Index 20 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA	328	1.4941690962099126	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA	320	1.4577259475218658	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA	250	1.1388483965014577	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA	205	0.9338556851311953	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC	174	0.7926384839650146	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG	165	0.7516399416909622	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA	135	0.6149781341107872	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA	123	0.5603134110787172	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	118	0.5375364431486881	RNA PCR Primer, Index 38 (96% over 27bp)
CAGTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	113	0.514759475218659	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA	110	0.5010932944606414	RNA PCR Primer, Index 33 (96% over 28bp)
GCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA	109	0.49653790087463556	RNA PCR Primer, Index 33 (96% over 28bp)
AGCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	101	0.4600947521865889	RNA PCR Primer, Index 20 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA	73	0.33254373177842567	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA	69	0.31432215743440234	RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA	69	0.31432215743440234	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC	68	0.3097667638483965	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA	64	0.2915451895043732	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA	62	0.2824344023323615	RNA PCR Primer, Index 20 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG	60	0.27332361516034986	RNA PCR Primer, Index 33 (96% over 28bp)
CAGATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	56	0.25510204081632654	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA	54	0.2459912536443149	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC	49	0.2232142857142857	RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT	49	0.2232142857142857	RNA PCR Primer, Index 33 (96% over 28bp)
CAGTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA	48	0.21865889212827988	RNA PCR Primer, Index 33 (96% over 28bp)
NTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	44	0.20043731778425655	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	42	0.1913265306122449	RNA PCR Primer, Index 38 (96% over 27bp)
CAGCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	41	0.18677113702623907	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAA	39	0.1776603498542274	RNA PCR Primer, Index 33 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGA	38	0.1731049562682216	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA	36	0.16399416909620992	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA	36	0.16399416909620992	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA	36	0.16399416909620992	RNA PCR Primer, Index 20 (96% over 29bp)
GACAGATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	35	0.15943877551020408	RNA PCR Primer, Index 20 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACA	35	0.15943877551020408	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG	34	0.15488338192419826	RNA PCR Primer, Index 20 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA	34	0.15488338192419826	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACA	34	0.15488338192419826	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	34	0.15488338192419826	RNA PCR Primer, Index 38 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA	33	0.15032798833819241	RNA PCR Primer, Index 33 (96% over 28bp)
GACAGACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	33	0.15032798833819241	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA	32	0.1457725947521866	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAG	30	0.13666180758017493	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAC	30	0.13666180758017493	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA	29	0.1321064139941691	RNA PCR Primer, Index 20 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAA	29	0.1321064139941691	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGG	27	0.12299562682215745	RNA PCR Primer, Index 33 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAA	25	0.11388483965014577	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACG	25	0.11388483965014577	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA	25	0.11388483965014577	RNA PCR Primer, Index 38 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	24	0.10932944606413994	RNA PCR Primer, Index 27 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAAAA	23	0.10477405247813411	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAA	23	0.10477405247813411	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC	23	0.10477405247813411	RNA PCR Primer, Index 33 (96% over 28bp)
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAA	22	0.10021865889212828	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACAAA	22	0.10021865889212828	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAAAA	22	0.10021865889212828	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACAA	22	0.10021865889212828	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGAAA	22	0.10021865889212828	RNA PCR Primer, Index 33 (96% over 28bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content