EGAF00002149538 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00002149538
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25053
Sequences flagged as poor quality	0
Sequence length	75
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	4244	16.940087015527084	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA	949	3.787969504650142	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	939	3.7480541252544604	RNA PCR Primer, Index 20 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA	317	1.2653175268430925	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA	285	1.137588312776913	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA	262	1.0457829401668461	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA	244	0.9739352572546203	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC	203	0.8102822017323273	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG	164	0.6546122220891709	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA	138	0.5508322356604	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	133	0.5308745459625593	RNA PCR Primer, Index 38 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA	103	0.4111284077755159	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA	91	0.3632299525006985	RNA PCR Primer, Index 33 (96% over 28bp)
GCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA	87	0.3472638007424261	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT	84	0.3352891869237217	RNA PCR Primer, Index 33 (96% over 28bp)
CAGTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	82	0.32730611104458546	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA	81	0.3233145731050174	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA	71	0.2833991937093362	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA	69	0.2754161178302	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA	65	0.2594499660719275	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC	58	0.2315092004949507	RNA PCR Primer, Index 20 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58	0.2315092004949507	No Hit
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC	58	0.2315092004949507	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA	57	0.2275176625553826	RNA PCR Primer, Index 20 (96% over 29bp)
AGCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	57	0.2275176625553826	RNA PCR Primer, Index 20 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG	54	0.21554304873667826	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA	50	0.1995768969784058	RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	49	0.19558535903883767	RNA PCR Primer, Index 38 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA	47	0.18760228315970143	RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA	47	0.18760228315970143	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA	47	0.18760228315970143	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA	45	0.17961920728056519	RNA PCR Primer, Index 33 (96% over 28bp)
NTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	45	0.17961920728056519	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA	44	0.17562766934099708	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGG	41	0.16365305552229273	RNA PCR Primer, Index 33 (96% over 28bp)
CAGTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA	40	0.15966151758272462	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA	39	0.15566997964315651	RNA PCR Primer, Index 20 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA	38	0.15167844170358838	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAA	37	0.14768690376402027	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	37	0.14768690376402027	RNA PCR Primer, Index 38 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG	36	0.14369536582445216	RNA PCR Primer, Index 20 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAA	36	0.14369536582445216	RNA PCR Primer, Index 33 (96% over 28bp)
CAGATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	34	0.13571228994531595	RNA PCR Primer, Index 33 (96% over 28bp)
CAGCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	33	0.13172075200574782	RNA PCR Primer, Index 33 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGA	33	0.13172075200574782	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC	32	0.1277292140661797	RNA PCR Primer, Index 33 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACA	32	0.1277292140661797	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA	31	0.12373767612661157	RNA PCR Primer, Index 38 (96% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30	0.11974613818704347	No Hit
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAA	29	0.11575460024747534	RNA PCR Primer, Index 33 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	27	0.10777152436833913	RNA PCR Primer, Index 33 (96% over 28bp)
GACAGATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	26	0.10377998642877101	RNA PCR Primer, Index 20 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content