EGAF00002149540 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00002149540
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12110
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	1872	15.45829892650702	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA	481	3.971924029727498	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	438	3.616845582163501	RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA	135	1.1147811725846408	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA	133	1.0982658959537572	RNA PCR Primer, Index 33 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA	133	1.0982658959537572	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA	105	0.8670520231213872	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC	84	0.6936416184971098	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG	83	0.685383980181668	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA	69	0.569777043765483	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA	66	0.5450041288191577	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	61	0.5037159372419487	RNA PCR Primer, Index 38 (96% over 27bp)
CAGTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	59	0.4872006606110652	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA	49	0.40462427745664736	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA	46	0.37985136251032203	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA	44	0.3633360858794385	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA	43	0.3550784475639967	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA	39	0.3220478943022296	RNA PCR Primer, Index 20 (96% over 29bp)
GCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA	37	0.305532617671346	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT	36	0.2972749793559042	RNA PCR Primer, Index 33 (96% over 28bp)
AGCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	33	0.27250206440957886	RNA PCR Primer, Index 20 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC	30	0.2477291494632535	RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	27	0.22295623451692817	RNA PCR Primer, Index 38 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC	27	0.22295623451692817	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA	27	0.22295623451692817	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG	25	0.20644095788604458	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA	24	0.19818331957060278	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA	23	0.18992568125516102	RNA PCR Primer, Index 20 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACA	23	0.18992568125516102	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG	22	0.18166804293971925	RNA PCR Primer, Index 20 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA	21	0.17341040462427745	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA	21	0.17341040462427745	RNA PCR Primer, Index 20 (96% over 29bp)
NTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	21	0.17341040462427745	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA	20	0.16515276630883566	RNA PCR Primer, Index 33 (96% over 28bp)
CAGCTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	20	0.16515276630883566	RNA PCR Primer, Index 33 (96% over 28bp)
CAGATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	20	0.16515276630883566	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACA	19	0.1568951279933939	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	19	0.1568951279933939	RNA PCR Primer, Index 38 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA	17	0.14037985136251033	RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGG	17	0.14037985136251033	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAA	17	0.14037985136251033	RNA PCR Primer, Index 33 (96% over 28bp)
CAGTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA	16	0.13212221304706853	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAAAA	16	0.13212221304706853	RNA PCR Primer, Index 33 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAA	16	0.13212221304706853	RNA PCR Primer, Index 33 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACAAA	15	0.12386457473162675	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA	15	0.12386457473162675	RNA PCR Primer, Index 20 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA	14	0.11560693641618498	RNA PCR Primer, Index 33 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCATTGGCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	14	0.11560693641618498	RNA PCR Primer, Index 27 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGA	14	0.11560693641618498	RNA PCR Primer, Index 33 (96% over 28bp)
GACAGATACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	13	0.10734929810074319	RNA PCR Primer, Index 20 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCATTGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACAA	13	0.10734929810074319	RNA PCR Primer, Index 33 (96% over 28bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content