FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118968
Sequences flagged as poor quality0
Sequence length75
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18471.552518324255262No Hit
AGTATCACTATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTTCTAGTGTAGATCTCGGTGGTCGCCGT12801.0759195750117678Illumina Single End PCR Primer 1 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.8725035303611055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.6110886961199651No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.39254253244569964No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.1521417524040078No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.13785219554838277No Hit
CTTATACACATCTGACGCTGCCGACGACTCTTCTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAA1470.12356263869275771Illumina Single End PCR Primer 1 (96% over 31bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.12356263869275771No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)