FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133928
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34412.5692909623081057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20781.5515799534078012No Hit
ATAGTGATACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCT16021.1961651036377756TruSeq Adapter, Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA14561.0871513051789021TruSeq Adapter, Index 7 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.9661907890807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9460.7063496804253031No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.34869482109790334No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.31136132847500153No Hit
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4080.3046412998028792TruSeq Adapter, Index 7 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.27178782629472553No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.2650677976226032No Hit
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3150.23520100352428172TruSeq Adapter, Index 7 (96% over 29bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG2200.16426736754076818No Hit
ACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1940.14485395137685922RNA PCR Primer, Index 7 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1590.1187205065408279No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT1450.10826712860641538No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.10677378890149931No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.10453377934412521No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.1022937697867511No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)