FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186591
Sequences flagged as poor quality0
Sequence length75
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA94025.038828239304147TruSeq Adapter, Index 7 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA41682.233762614488373TruSeq Adapter, Index 7 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA38692.073519087201419TruSeq Adapter, Index 7 (96% over 29bp)
ATAGTGATACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCT31111.6672829879254627TruSeq Adapter, Index 7 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA15540.8328375966686495RNA PCR Primer, Index 7 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA14570.7808522383180325RNA PCR Primer, Index 7 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.4850180340959639No Hit
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA7930.42499370280452964TruSeq Adapter, Index 7 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA6450.34567583645513444TruSeq Adapter, Index 7 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA5800.31084028704492717TruSeq Adapter, Index 7 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.2743969430465564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.2465285035183905No Hit
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA4200.22509124234287828TruSeq Adapter, Index 7 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC4180.22401937928410265TruSeq Adapter, Index 7 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG3750.20097432352042702TruSeq Adapter, Index 7 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA3310.17739333622736358TruSeq Adapter, Index 7 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA3220.17256995246287335TruSeq Adapter, Index 7 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG3200.17149808940409775TruSeq Adapter, Index 7 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA3180.17042622634532212Illumina PCR Primer Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA3140.16828250022777091TruSeq Adapter, Index 7 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA2890.15488421199307578TruSeq Adapter, Index 7 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA2870.15381234893430015TruSeq Adapter, Index 7 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA2850.15274048587552455TruSeq Adapter, Index 7 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC2770.14845303364042212TruSeq Adapter, Index 7 (96% over 29bp)
CAGTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2630.14094999222899282RNA PCR Primer, Index 7 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC2570.137734403052666TruSeq Adapter, Index 7 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG2520.13505474540572696TruSeq Adapter, Index 7 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA2240.12004866258286842RNA PCR Primer, Index 7 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.11790493646531719No Hit
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAA2060.11040189505388792TruSeq Adapter, Index 7 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA2050.10986596352450011TruSeq Adapter, Index 7 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA1950.10450664823062207TruSeq Adapter, Index 7 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)