FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428217
Sequences flagged as poor quality0
Sequence length75
%GC33

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78861.8415896613165756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44501.0391927457340555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33320.7781101637721062No Hit
AGTATCACTATCTGTCTCTTATACACATCTGACGCTGCCGACGAACCATGGAGTGTAGATCTCGGTGGTCGCCGT18720.43716153258744983Illumina Single End PCR Primer 1 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18030.4210482068670791No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.1655702599383023No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.11909849445491422No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)