FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360126
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA68971.9151630262741373TruSeq Adapter, Index 7 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66911.8579608248224233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39661.1012812182402825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.8388730610952834No Hit
ATAGTGATACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCT28630.7949995279429977TruSeq Adapter, Index 7 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA20640.5731327368754269TruSeq Adapter, Index 7 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA18420.51148764599057TruSeq Adapter, Index 7 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17060.4737230858088558No Hit
TATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA7270.201873788618428RNA PCR Primer, Index 7 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA6880.19104424562514233RNA PCR Primer, Index 7 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.1710512431759995No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.15022519895814243No Hit
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA5260.146059990114571TruSeq Adapter, Index 7 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA4090.11357136113471396TruSeq Adapter, Index 7 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)