FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500305
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105122.101118317826126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61061.2204555221314999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51431.0279729365087298No Hit
ATAGTGATACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCT49440.988197199708178TruSeq Adapter, Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA29830.5962362958595256TruSeq Adapter, Index 7 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.5680534873727027No Hit
TTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA10560.21107124653961082TruSeq Adapter, Index 7 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9390.18768551183777896No Hit
ATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA8260.1650992894334456TruSeq Adapter, Index 7 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.14890916540910046No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.10833391631105026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT5190.10373672060043372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT5050.1009384275591889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)