FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392533
Sequences flagged as poor quality0
Sequence length75
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66261.688010944302772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38250.9744403655234083No Hit
AGTATCACTATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTGATAGGTGTAGATCTCGGTGGTCGCCGT26610.6779047876229514Illumina Single End PCR Primer 1 (100% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22600.5757477715249418No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14720.3750003184445639No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.18062175664211672No Hit
CTTATACACATCTGACGCTGCCGACGATGTGATAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAA4110.1047045726091819Illumina Single End PCR Primer 1 (100% over 29bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.10343079435359574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)