FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310670
Sequences flagged as poor quality0
Sequence length75
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35441.140760292271542No Hit
AGTATCACTATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAGGCCTGGTGTAGATCTCGGTGGTCGCCGT26390.8494544049956545Illumina RNA PCR Primer (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20530.6608298194225384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13780.4435574725593073No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.20665014323880648No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.10686580616087811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)