FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250074
Sequences flagged as poor quality0
Sequence length75
%GC25

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52192.086982253253037No Hit
CTTATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA33511.3400033590057343Illumina PCR Primer Index 12 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32811.3120116445532122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22780.9109303646120748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.571830738101522No Hit
ATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA9360.3742892103937234Illumina PCR Primer Index 12 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA6570.2627222342186713RNA PCR Primer, Index 32 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.24952614026248232No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5640.22553324216032056No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.204339515503411No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.14795620496333087No Hit
ACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA3440.1375592824523941Illumina PCR Primer Index 12 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3400.13595975591224999No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2670.10676839655461984No Hit
TATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2590.10356934347433161Illumina PCR Primer Index 12 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA2530.10117005366411541Illumina PCR Primer Index 12 (96% over 27bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)