FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233532
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77283.3091824674991006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48742.0870801431923676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30431.303033417261874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24571.0521042084168337No Hit
CTTATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22680.9711731154616926Illumina PCR Primer Index 12 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.6881283935392152No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13030.5579535138653375No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.36954250381104087No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.336570577051539No Hit
ATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7080.3031704434510046Illumina PCR Primer Index 12 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5570.23851121045509824RNA PCR Primer, Index 32 (96% over 29bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.1331723275611051No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.12589281126355276No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.11946970864806536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2520.10790812394018806No Hit
ACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2470.1057670897350256Illumina PCR Primer Index 12 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2430.10405426237089564No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.10105681448366818No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)