FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314730
Sequences flagged as poor quality0
Sequence length75
%GC22

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99533.1623931623931623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59811.9003590379055062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38761.231531789152607No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26170.8315063705398277No Hit
CTTATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA24530.7793982143424523Illumina PCR Primer Index 12 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13610.4324341499062688No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11980.3806437263686334No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.31074254122581263No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.27070822609856066No Hit
ATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6510.20684396149080164Illumina PCR Primer Index 12 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT4820.15314714199472565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT4560.14488609284148318No Hit
TTATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3660.11629015346487466RNA PCR Primer, Index 32 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT3620.11501922282591427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTT3390.1077113716518921No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.10421631239475104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)