FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002149624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952925
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138661.4550987748248814No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG95881.0061652281134401No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC88460.928299708791353No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG56210.5898680378833591No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG47700.5005640527848467No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGT40870.4288899965894483No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT40820.4283652963244747No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC32900.345252774352651No Hit
GATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGC31050.3258388645486266No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT29680.31146207728834907No Hit
ACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATAT27650.2901592465304195No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG25040.2627698926987958No Hit
CACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTA25010.26245507253981165No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC23550.2471338248025815No Hit
CCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACG20500.21512710863918985No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20210.2120838471023428No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT20160.21155914683736915No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT20100.2109295065194008No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC19570.20536768371068026No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT19170.2011700815908912No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT17310.18165123173387204No Hit
ATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT16990.17829315003804078No Hit
ATGGGTAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA16900.17734868956108824No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG16900.17734868956108824No Hit
GATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAAT16850.1768239892961146No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG16070.1686386651625259No Hit
CTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCG15270.16024346092294778No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA15140.15887924023401634No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC14620.15342235747829053No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14460.1517433166303749No Hit
TGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCC14350.1505889760474329No Hit
TTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCC14070.14765065456358054No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG13900.1458666736626702No Hit
TGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCC12770.1340084476742661No Hit
ACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG12490.13107012619041372No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCA12330.1293910853424981No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT11880.12466878295773538No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGT11860.12445890285174593No Hit
GGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACA11500.12068106094393577No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT11270.11826743972505706No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA11230.11784767951307815No Hit
TTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA10900.11438465776425218No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC10870.114069837605268No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC10780.11312537712831545No Hit
AATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG9790.10273631188183749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)