FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002149625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952925
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC107371.1267413490043814No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG95371.0008132854107092No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG62080.6514678489912638No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG57500.6034053047196789No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGT44190.4637300941836976No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT41640.43697038067004224No Hit
CACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTA39440.4138835690112024No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC37140.3897473568224152No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT33520.3517590576383241No Hit
GATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGC32870.34493795419366685No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG27560.28921478605346695No Hit
ACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATAT27460.2881653855235197No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT23870.2504919064984128No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT23570.24734370490857097No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT23370.24524490384867642No Hit
GATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAAT22720.23842380040401923No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT22530.2364299393971194No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC21880.22960883595246215No Hit
ATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT21320.22373219298475744No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20770.2179604900700475No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC20630.21649132932812132No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG19240.20190466196185428No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG19180.20127502164388592No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG18160.1905711362384238No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC17680.1855340136946769No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG17190.1803919510979353No Hit
TGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCC16490.17304614738830443No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA15950.16737938452658918No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA14890.15625573890914815No Hit
CCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACG14820.15552115853818504No Hit
ACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG14120.14817535482855418No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13620.14292835217881786No Hit
ATGGGTAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA13550.14219377180785475No Hit
TTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCC13380.14040979090694442No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT13200.13852086995303933No Hit
CTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCG12870.13505784820421335No Hit
GGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACA12790.13421832778025553No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGT12660.1328541070913241No Hit
TGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCC12310.12918120523650864No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC12300.12907626518351392No Hit
AATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG12000.12592806359367212No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC11910.12498360311671958No Hit
TGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT11850.12435396279875122No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG11610.12183540152687776No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT11550.12120576120890941No Hit
TAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTG11530.12099588110291996No Hit
ATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAA11430.1199464805729727No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT11020.11564393840018888No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC10660.11186609649237872No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT10530.11050187580344728No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCA10470.1098722354854789No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC10310.1081931946375633No Hit
CACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTA9960.10452029278274785No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT9930.10420547262376369No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC9910.10399559251777424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)