FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21762
Sequences flagged as poor quality0
Sequence length20-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4492.063229482584321No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.9190331770976933No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.5008730815182428No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.45951658854884664No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC840.38599393438103113No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.3078761143277272No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.2527341237018656No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT540.24813895781637718Clontech Universal Primer Mix Long (96% over 26bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC480.22056796250344637No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA480.22056796250344637No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG460.21137763073246943No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.20678246484698098No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA390.17921146953405018No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT390.17921146953405018No Hit
ATGGGAAGCAGTGGTATCAACGC360.1654259718775848No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT360.1654259718775848No Hit
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.16083080599209632No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC340.15623564010660784No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC320.1470453083356309No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1470453083356309No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC300.13785497656465398No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA290.1332598106791655No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG290.1332598106791655No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC280.12866464479367706No Hit
ATGGGGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.12866464479367706No Hit
ATGGATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.12406947890818859No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG270.12406947890818859No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT270.12406947890818859No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG260.11947431302270012No Hit
ATACATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11487914713721166No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT250.11487914713721166No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA240.11028398125172319No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT240.11028398125172319No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG240.11028398125172319Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA240.11028398125172319No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC240.11028398125172319No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC230.10568881536623471No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG230.10568881536623471No Hit
ATGGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10109364948074626No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT220.10109364948074626No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10109364948074626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)