FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133002
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG29982.2541014420835777Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG26421.9864362941910647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT16881.2691538473105668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11050.8308145742169291Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.7511165245635404No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9500.714274973308672Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8640.6496142915144133Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG8420.6330731868693704Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC8240.6195395557961534Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6960.5233004014977218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6610.4969850077442444Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6530.49097006060059245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGG6420.482699508278071No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5750.43232432594998577Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT5630.4233019052345077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.40525706380355186No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG5050.37969353844303094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.36615990736981396No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT4700.35337814468955353No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4250.31954406700651117Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG4230.3180403302205982No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4200.31578472504172866Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGA3740.28119877896572987No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCA3460.26014646396294794No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAG3450.25939459556999145No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGG3350.25187591164042644No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3230.2428534909249485Clontech Universal Primer Mix Long (96% over 25bp)
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGC3040.22856799145877507No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAACCAGTTAAAAT3010.22631238627990558No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT2900.21804183395738413No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA2790.2097712816348626No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2760.20751567645599314Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2620.19698951895460218Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2620.19698951895460218No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2610.19623765056164566Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2520.18947083502503723Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC2360.17744094073773325No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2350.17668907234477677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGGACACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGA2290.1721778619870378No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC2270.1706741252011248No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2250.1691703884152118Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTT2210.1661629148433858No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCG2140.16089983609269032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.16089983609269032No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2110.15864423091382085Clontech Universal Primer Mix Long (96% over 26bp)
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT2050.15413302055608186No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG2030.15262928377016888Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT2020.1518774153772124Clontech Universal Primer Mix Long (96% over 26bp)
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGG2000.15037367859129938No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTC2000.15037367859129938No Hit
GCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCA1980.1488699418053864No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1980.1488699418053864Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG1930.1451105998406039No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTG1810.13608817912512594No Hit
GCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCTCCCCAAGGCCCAACGCACCCAA1810.13608817912512594No Hit
GAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA1800.13533631073216945No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT1770.13308070555329995No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGT1760.13232883716034347No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA1740.13082510037443046Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT1720.12932136358851748Clontech SMART CDS Primer II A (100% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT1650.124058284837822Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC1630.122554548051909No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1620.12180267965895249Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT1610.121050811265996Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1570.11804333769417001Clontech Universal Primer Mix Long (96% over 26bp)
CCCCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC1570.11804333769417001No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC1520.11428399572938754Clontech Universal Primer Mix Long (96% over 26bp)
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG1520.11428399572938754No Hit
ATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC1510.11353212733643103No Hit
CTCTAAGGGTTGGGTGCGTTGGGCCTTGGGGAGACGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCC1500.11278025894347454No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT1490.11202839055051803Clontech Universal Primer Mix Long (96% over 25bp)
TGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCT1480.11127652215756154No Hit
GTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTG1470.11052465376460503No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT1440.10826904858573555Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT1430.10751718019277906No Hit
GCCTTGGGGAGACGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCCTTTCAGCGCTGGGGTGCTGACA1410.10601344340686607No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA1400.10526157501390956No Hit
GGATATGGATTCTCCGCGGCAACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCT1400.10526157501390956No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATGTACCTCCCATTTAGAGCTGCATT1390.10450970662095307No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG1390.10450970662095307No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1380.10375783822799657Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.10375783822799657No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA1370.10300596983504008Clontech SMART CDS Primer II A (96% over 26bp)
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGC1350.10150223304912709No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGG1350.10150223304912709No Hit
ACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA1340.10075036465617057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)