FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133002
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG21441.6120058344987291Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG19161.440579840904648Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11780.8857009669027534Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7850.5902166884708501Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7450.5601419527525903Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6270.4714214823837236Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5730.43082058916407273Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.4293168523781597No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC5610.4217981684485948Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5570.4187906948767688Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG5290.39773837987398686No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5270.3962346430880738Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGG5190.3902196959444219No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT4630.3481150659388581No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4490.3375889084374671Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4350.3270627509360761Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT3960.2977398836107728No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3350.25187591164042644No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3340.25112404324746995Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.23834228056720952No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2980.22405678110103605Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCA2830.2127787552066886No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAG2810.21127501842077562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.20150072931234114No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGA2670.20074886091938468No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT2570.1932301769898197No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2550.19172644020390672Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2440.18345588788138525Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA2430.18270401948842874No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC2430.18270401948842874No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGC2370.17819280913068977No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAACCAGTTAAAAT2370.17819280913068977No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGG2290.1721778619870378No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2250.1691703884152118Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTT2200.1654110464504293No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2090.15714049412790784No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2080.15638862573495135Clontech SMART CDS Primer II A (96% over 26bp)
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCG2080.15638862573495135No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT2010.15112554698425587No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT1950.14661433662651688No Hit
GTTGGACACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGA1840.1383437843039954No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1830.13759191591103892Clontech Universal Primer Mix Long (96% over 26bp)
GCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCA1760.13232883716034347No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG1740.13082510037443046No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1730.13007323198147397Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1710.12856949519556096Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG1600.1202989428730395Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTAAGGGTTGGGTGCGTTGGGCCTTGGGGAGACGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCC1570.11804333769417001No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1560.11729146930121352Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC1540.11578773251530053No Hit
GCCTTGGGGAGACGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCCTTTCAGCGCTGGGGTGCTGACA1530.11503586412234403No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1510.11353212733643103Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAG1490.11202839055051803No Hit
GCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCTCCCCAAGGCCCAACGCACCCAA1490.11202839055051803No Hit
CTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAGTTTTCATGCCAGGTCGTACTCATAACCGCAGCAGGTCT1490.11202839055051803No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTG1470.11052465376460503No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG1430.10751718019277906Clontech Universal Primer Mix Long (96% over 26bp)
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT1400.10526157501390956No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGGGGAGACGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGC1390.10450970662095307No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1380.10375783822799657Clontech Universal Primer Mix Long (96% over 26bp)
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGG1370.10300596983504008No Hit
TTTCAGCGCTGGGGTGCTGACACCCTTGGCAGGCTTCGGCCGTCCGGCGCACGTTTAACAACCAACTTAGAACTG1350.10150223304912709No Hit
GAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA1340.10075036465617057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)