FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288667
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTGTTCGCTATCGATCTCTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCC53481.8526537498224596No Hit
GCATATGCGTGTGCGAGTTGTAGGGTTTGAAGTTACGACAAGTGGGTTGTTTTGCATAAATTCCTTGGAAAGGGA48551.6818687276342639No Hit
CTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTGCATTTACAAGACTATCGCTTTCTACG43671.5128158050625808No Hit
CCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCATCCCTGAATCTCACATGCATATGCGTGTGCGAGTTGTAGG42641.47713455296241No Hit
CCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTGCATTTACAAGACTATCGCTTTCTACGGT32471.1248254909636364No Hit
CGCATATGCATGTGAGATTCAGGGATGAGCTTCTCCCGTTTCAGTCGCCCTTACTAAGGGAATCCCTATTGGTTT24190.8379897944690595No Hit
TAGTAAGGGCGACTGAAACGGGAGAAGCTCATCCCTGAATCTCACATGCATATGCGTGTGCGAGTTGTAGGGTTT23570.8165117592242964No Hit
CTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTTA22280.7718235891182573No Hit
GTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTTAGT18340.6353341393370215No Hit
GTACATGGGACGGGCATCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAAC17000.5889138696144692No Hit
ACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTGCATTTA16550.573324973065851No Hit
GTATCAACGCAGAGTACATGGGACGGGCATCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCG14720.5099301271014698No Hit
CGGTACTTGTTCGCTATCGATCTCTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACT13450.465934796842036No Hit
ATGCATATGCGTGTGCGAGTTGTAGGGTTTGAAGTTACGACAAGTGGGTTGTTTTGCATAAATTCCTTGGAAAGG12790.4430710819040624No Hit
ACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTGCATTTACAAGACT11480.3976900719514181No Hit
TCCCTGAATCTCACATGCATATGCGTGTGCGAGTTGTAGGGTTTGAAGTTACGACAAGTGGGTTGTTTTGCATAA11400.39491871256499705No Hit
CATCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCC11380.39422587271839177No Hit
GGCATCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTC10500.36374091946776044No Hit
TTACAAGACTATCGCTTTCTACGGTTTCCCTTTCCAAGGAATTTATGCAAAACAACCCACTTGTCGTAACTTCAA10380.3595838803881289No Hit
ACGCAGAGTACATGGGACGGGCATCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAA10110.3502305424589579No Hit
GCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTGCATTTACAAGACTATCGC10050.3481520229191421No Hit
AACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTT10020.3471127631492342No Hit
ATCTCTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTA9880.34226288422299744No Hit
GCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTGCATTTACAAGACTATCGCTTTCT9620.33325596621712905No Hit
GCCTCTAGACGATAAAATAATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGGTTGGCT9520.3297917669841028No Hit
TATCAACGCAGAGTACATGGGACGGGCATCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGG9140.3166278098986029No Hit
GAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAG8410.291339155497511No Hit
CTATCGATCTCTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCG8410.291339155497511No Hit
GTTGTAGGGTTTGAAGTTACGACAAGTGGGTTGTTTTGCATAAATTCCTTGGAAAGGGAAACCGTAGAAAGCGAT7970.2760966788721953No Hit
ATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTGCA7870.272632479639169No Hit
CTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTGCATTTACAAGACTATCGCTTT7550.2615470420934849No Hit
AGTCGTAACCGTACCGATCTCCGCAACAGGTACCCAAGGTGAGCAGCCTCTAGACGATAAAATAATGTAGGTAAG7530.2608542022468796No Hit
GTCGTAACCGTACCGATCTCCGCAACAGGTACCCAAGGTGAGCAGCCTCTAGACGATAAAATAATGTAGGTAAGG7480.2591221026303665No Hit
TACTAAGGGAATCCCTATTGGTTTCTTTTCCTCCGCTTATTAATATGCTTAAGTTCAGCGGGTAGTCAATCATAA7390.2560043233206428No Hit
CTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTG7310.2532329639342218No Hit
GTTCGCTATCGATCTCTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCA7290.25254012408761656No Hit
CACGGTACTTGTTCGCTATCGATCTCTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCA7280.2521937041643139No Hit
ATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTTAGTAAG6970.2414546865419324No Hit
CTCTAAAAGTTTACGATTCATGAGCCGACTTTGCATTTACAAGACTATCGCTTTCTACGGTTTCCCTTTCCAAGG6840.23695122753899822No Hit
CATATGCATGTGAGATTCAGGGATGAGCTTCTCCCGTTTCAGTCGCCCTTACTAAGGGAATCCCTATTGGTTTCT6730.23314060838266928No Hit
GCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTT6660.23071566891955092No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6550.226905049763222Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.22413369037680098No Hit
CCCTACAACTCGCACACGCATATGCATGTGAGATTCAGGGATGAGCTTCTCCCGTTTCAGTCGCCCTTACTAAGG6450.2234408505301957No Hit
CACATGCATATGCGTGTGCGAGTTGTAGGGTTTGAAGTTACGACAAGTGGGTTGTTTTGCATAAATTCCTTGGAA6280.21755171183405103No Hit
CCAATAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCATCCCTGAATCTCACATGCATATGCGTGT6140.2127018329078142No Hit
TCACGGTACTTGTTCGCTATCGATCTCTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGC5810.20126997543882744No Hit
GGTATCAACGCAGAGTACATGGGACGGGCATCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGC5580.19330231720286697No Hit
ATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGA5520.1912237976630512No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG5490.19018453789314332Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCATATGCGTGTGCGAGTTGTAGGGTTTGAAGTTACGACAAGTGGGTTGTTTTGCATAAATTCCTTGGAAAGGG5440.18845243827663016No Hit
TTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCATCCCTG5210.1804847800406697No Hit
GCTTGGGAGTGCAGCGCAAAGTGGGAGGTAAATAGCTTCTAAAGCTAAATATTAGTTAGAGATCGATAGCGAACA5020.17390280149791976No Hit
ACTTTGCATTTACAAGACTATCGCTTTCTACGGTTTCCCTTTCCAAGGAATTTATGCAAAACAACCCACTTGTCG4860.1683600827250777No Hit
GAGGAAAAGAAACCAATAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCATCCCTGAATCTCACAT4790.1659351432619593No Hit
TTCTACGGTTTCCCTTTCCAAGGAATTTATGCAAAACAACCCACTTGTCGTAACTTCAAACCCTACAACTCGCAC4770.16524230341535404No Hit
GTCTTGTAAATGCAAAGTCGGCTCATGAATCGTAAACTTTTAGAGTCGCGTTGCTTGGGAGTGCAGCGCAAAGTG4760.1648958834920514No Hit
GGAGAAGCTCATCCCTGAATCTCACATGCATATGCGTGTGCGAGTTGTAGGGTTTGAAGTTACGACAAGTGGGTT4630.16039242448911722No Hit
ACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAA4610.15969958464251197No Hit
GGTTTGAAGTTACGACAAGTGGGTTGTTTTGCATAAATTCCTTGGAAAGGGAAACCGTAGAAAGCGATAGTCTTG4590.1590067447959067No Hit
CTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTA4540.15727464517939357No Hit
TTGCTTGGGAGTGCAGCGCAAAGTGGGAGGTAAATAGCTTCTAAAGCTAAATATTAGTTAGAGATCGATAGCGAA4530.15692822525609093No Hit
ATCGATCTCTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGAC4420.15311760609976202No Hit
GTCTAAACCCAGCTCACGTTCCCTTTTAACGGGTGAACAATCCAACACTTGGCGAATGCTGCTTCGCAATGATAG4400.15242476625315673No Hit
ATGCAAAACAACCCACTTGTCGTAACTTCAAACCCTACAACTCGCACACGCATATGCATGTGAGATTCAGGGATG4340.150346246713341No Hit
CTTCAAACCCTACAACTCGCACACGCATATGCATGTGAGATTCAGGGATGAGCTTCTCCCGTTTCAGTCGCCCTT4170.14445710801719627No Hit
GCTTAGGAGTGTGTAACAACTCACCTGCCGAATGGACCGGCCCCGAAAATGGATGGCGCTAAAATGTAATGTCGA4120.14272500840068314No Hit
GTTTTGCATAAATTCCTTGGAAAGGGAAACCGTAGAAAGCGATAGTCTTGTAAATGCAAAGTCGGCTCATGAATC4110.14237858847738052No Hit
GAGTACATGGGACGGGCATCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAA4110.14237858847738052No Hit
ATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCATCC4090.14168574863077527No Hit
ATCCAACACTTGGCGAATGCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTA4090.14168574863077527No Hit
CCCTTACTAAGGGAATCCCTATTGGTTTCTTTTCCTCCGCTTATTAATATGCTTAAGTTCAGCGGGTAGTCAATC3950.13683586970453843No Hit
TTCATGAGCCGACTTTGCATTTACAAGACTATCGCTTTCTACGGTTTCCCTTTCCAAGGAATTTATGCAAAACAA3930.1361430298579332No Hit
GTGTGTACAAAGGGCAGGGACGTAATCGACGTGGTTTAGTGAACCACGACTACTAGAAATTCCTCGTTCAAGACA3920.13579660993463055No Hit
ATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCATCCCT3830.1326788306249069No Hit
ATTCAGGGATGAGCTTCTCCCGTTTCAGTCGCCCTTACTAAGGGAATCCCTATTGGTTTCTTTTCCTCCGCTTAT3770.13060031108509113No Hit
ATTCATGAGCCGACTTTGCATTTACAAGACTATCGCTTTCTACGGTTTCCCTTTCCAAGGAATTTATGCAAAACA3730.1292146313918806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.12713611185206483No Hit
GCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGA3480.12055413330931489No Hit
CCTCTAGACGATAAAATAATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGGTTGGCTC3470.12020771338601226No Hit
GCTCATCCCTGAATCTCACATGCATATGCGTGTGCGAGTTGTAGGGTTTGAAGTTACGACAAGTGGGTTGTTTTG3430.11882203369280174No Hit
GTTCTGGGCCGCACGCGCGCTACAATGGTATGTGTAAAGCGTAAAAAACTTTACAGGAATGTCGGGTAAACCCGG3350.1160506743063807No Hit
CCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAGTTTACGATTCATGAGCCGACTTTGCATTTAC3320.11501141453647282No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3270.11327931491995967Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTT3270.11327931491995967No Hit
CCTTTAGGAATATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTATCAGTTCCAAATCAAT3160.10946869576363076No Hit
GTCCGTATCAGTTCCAAATCAATCGATAATTGTAAAGCTTATAAAAAAGTACTAAACCCTAGTACAAAATAACTC3140.10877585591702552No Hit
CTACTAGATAGCTCGATTAGTCTTTCGCCGCTATACTTGAATTTGACGATCGATTTGCACGTCAGAACCGCTTCG3130.10842943599372286No Hit
GACTATCGCTTTCTACGGTTTCCCTTTCCAAGGAATTTATGCAAAACAACCCACTTGTCGTAACTTCAAACCCTA3090.10704375630051234No Hit
ATCAACGCAGAGTACATGGGACGGGCATCTGTTATGATTGACTACCCGCTGAACTTAAGCATATTAATAAGCGGA3080.10669733637720973No Hit
TCTCTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAA3050.10565807660730185No Hit
CTAACTAATATTTAGCTTTAGAAGCTATTTACCTCCCACTTTGCGCTGCACTCCCAAGCAACGCGACTCTAAAAG3030.10496523676069658No Hit
CTTTGGAGCCAACCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTACCTACATTATTTTATCGTCT3030.10496523676069658No Hit
GCATATTAATAAGCGGAGGAAAAGAAACCAATAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAT3030.10496523676069658No Hit
GTGTTGGATTGTTCACCCGTTAAAAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCT2970.10288671722088082No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTTTTAACGGGTGAACAATCCAACACTTGGCGAATGCTGCTT2930.1015010375276703No Hit
GTCCTAAGATGAGCTCAATGAGGACAGAAACCTCATGTAGAGTAAAAGGGCAAAAGCTCATTTGATATTGATTTT2890.10011535783445978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)