FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323286
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38081.1779043942515297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.7374275409389828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.42841323162772277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.3155101056030883No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA8660.26787426612968085RNA PCR Primer, Index 41 (96% over 30bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCCCCGCTGTCTC6490.20075103778078854No Hit
CATTTAAGGAACTCGGCAAAATACCTCCGTAAGTTCGCGAGAAGGAGGCCCGATTGGTGCGCAAGCATTGGTCGG6340.19611118328662544No Hit
CCACAAGACCGTACCCTAAACCGACACAGGTGGACTGGTAGAGAATACCAAGGCGCTTGAGAGAACCACATTTAA6330.19580185965368127No Hit
GTCCACATTTGAGACAGCGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT6020.18621282703241093No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCAATTCAATGCTTGCACATCTCCTTTTAACCTT5760.17817041257586164No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.1688907035875355No Hit
TTCCGGCACCGGGCAGGCGTCAGACCCTATACGTCGCCTTACGGCTTCGCAGAGCCCTGTGTTTTTAGTAAACAG5120.15837370006743257No Hit
TTCACAGACAATTCAATTTCGCTGAGTCCACATTTGAGACAGCGGGGAAGTCGTTACGCCATTCGTGCAGGTCGG4730.14631007838260857No Hit
CATTTGAGACAGCGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTT4690.14507278385083178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4640.14352616568611076No Hit
GTCTTGTGGAGGGCTATTTCCAGGAACCGCTCAGCCGCCCTTCCAATCCGATAAGGAAGAACAACACCTGCGATC4520.13981428209078028No Hit
AACCAGGGGGTGGCGACTGTTTACTAAAAACACAGGGCTCTGCGAAGCCGTAAGGCGACGTATAGGGTCTGACGC4510.1395049584578361No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.13641172212839406No Hit
GTACATGGGGTCTTTCCGTCTAACCGCGGGAAGTCTGCATCTTCACAGACAATTCAATTTCGCTGAGTCCACATT4300.1330091621660078No Hit
GGGTGAGCCGGCCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAACCAGGTTAATAGTCCTGGGCCAGGAGA4140.12805998403890054No Hit
ACCCTATACGTCGCCTTACGGCTTCGCAGAGCCCTGTGTTTTTAGTAAACAGTCGCCACCCCCTGGTTTGTGCCC3880.12001756958235123No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTAACCGCGGGAAGTCTGCATCTTCACAGACAATTCAATTTCG3790.1172336568858534No Hit
CCCTATACGTCGCCTTACGGCTTCGCAGAGCCCTGTGTTTTTAGTAAACAGTCGCCACCCCCTGGTTTGTGCCCC3720.11506839145524396No Hit
CCTTTTAACCTTCCGGCACCGGGCAGGCGTCAGACCCTATACGTCGCCTTACGGCTTCGCAGAGCCCTGTGTTTT3710.11475906782229975No Hit
CCCCTGGTTTGTGCCCCCGACCAATGCTTGCGCACCAATCGGGCCTCCTTCTCGCGAACTTACGGAGGTATTTTG3690.11414042055641134No Hit
GCCCTGTGTTTTTAGTAAACAGTCGCCACCCCCTGGTTTGTGCCCCCGACCAATGCTTGCGCACCAATCGGGCCT3300.10207679887158738No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)