FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323286
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33951.0501537338455733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.6916476432632406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.36252729781060733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.2780819460168396No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCCCCGCTGTCTC6980.21590789579505454No Hit
CATTTAAGGAACTCGGCAAAATACCTCCGTAAGTTCGCGAGAAGGAGGCCCGATTGGTGCGCAAGCATTGGTCGG6440.1992044196160675No Hit
GTCCACATTTGAGACAGCGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT4980.1540431692062137No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCAATTCAATGCTTGCACATCTCCTTTTAACCTT4900.15156858014266006No Hit
TTCCGGCACCGGGCAGGCGTCAGACCCTATACGTCGCCTTACGGCTTCGCAGAGCCCTGTGTTTTTAGTAAACAG4860.15033128561088324No Hit
GTATAGGGTCTGACGCCTGCCCGGTGCCGGAAGGTTAAAAGGAGATGTGCAAGCATTGAATTGAAGCCCCGGTAA4840.14971263834499482No Hit
CCACAAGACCGTACCCTAAACCGACACAGGTGGACTGGTAGAGAATACCAAGGCGCTTGAGAGAACCACATTTAA4640.14352616568611076No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.12960660220362155No Hit
GTCTTGTGGAGGGCTATTTCCAGGAACCGCTCAGCCGCCCTTCCAATCCGATAAGGAAGAACAACACCTGCGATC4120.12744133677301214No Hit
AACCAGGGGGTGGCGACTGTTTACTAAAAACACAGGGCTCTGCGAAGCCGTAAGGCGACGTATAGGGTCTGACGC4110.12713201314006792No Hit
GGGTGAGCCGGCCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAACCAGGTTAATAGTCCTGGGCCAGGAGA3850.11908959868351862No Hit
GTACATGGGGTCTTTCCGTCTAACCGCGGGAAGTCTGCATCTTCACAGACAATTCAATTTCGCTGAGTCCACATT3800.1175429805187976No Hit
TTCACAGACAATTCAATTTCGCTGAGTCCACATTTGAGACAGCGGGGAAGTCGTTACGCCATTCGTGCAGGTCGG3750.1159963623540766No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.11444974418935555No Hit
ACCCTATACGTCGCCTTACGGCTTCGCAGAGCCCTGTGTTTTTAGTAAACAGTCGCCACCCCCTGGTTTGTGCCC3660.11321244965757873No Hit
CATTTGAGACAGCGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTT3620.11197515512580193No Hit
GGTAGAGAATACCAAGGCGCTTGAGAGAACCACATTTAAGGAACTCGGCAAAATACCTCCGTAAGTTCGCGAGAA3500.10826327153047148No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTAACCGCGGGAAGTCTGCATCTTCACAGACAATTCAATTTCG3400.10517003520102942No Hit
CCCTATACGTCGCCTTACGGCTTCGCAGAGCCCTGTGTTTTTAGTAAACAGTCGCCACCCCCTGGTTTGTGCCCC3320.10269544613747579No Hit
CCTTTTAACCTTCCGGCACCGGGCAGGCGTCAGACCCTATACGTCGCCTTACGGCTTCGCAGAGCCCTGTGTTTT3320.10269544613747579No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.10083950433981057No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)