FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289884
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT23000.7934208166025031No Hit
GTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGG11360.3918808902871493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.3853265444108678No Hit
ACCCAGAAGCATCCTCTACAAATTACAACTCGGGCTTGCGCCAGATTTCAAATTTGAGCTGTTGCCGCTTCACTC11130.3839466821211243No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCGCGTTG10440.36014405762304924No Hit
GCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGCAAGCCCGAGTTGTAATTTGTAGA9560.32978708724869255No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACG9400.32426763808971865No Hit
ATCCTAAGCCATAGGGAAGTTCCGTTTCAAAGTGCGCGCTACGCGCCGTTTGGCGAAAGGGAAGCCGGTTAACAT9280.3201280512204882No Hit
GTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTA8520.293910667715362No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCCTCTACAAATTACAACTCGGGC8120.2801120448179272No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCCGGGGAATCTGCCCAAGGCCCAACGCGCCCAA7730.2666583874929282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.253894661312801No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA7340.25320473016792927No Hit
ACGTAAAGGGTGCGGGCCGGTCACATACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGATCGG7230.24941010887113468No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATCATTCCTTCCGGTGG7110.2452705220019042No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAAAGGGTGCGGGCCGGTCACATACGGGATTCTCACCCTCT7040.24285576299485312No Hit
CTTCTGGGTAGCGACCGATCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGTGACCGGCC6960.24009603841536614No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAAAGGGTGCGGGCCGGTCACATACGGG6880.23733631383587916No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCC6640.22905714009741826No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA6360.21939810406921392No Hit
GCACAAAGGTGTGCCGCTCTACCAGCTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTA6340.21870817292434214No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT6080.20973906804100953No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAA6060.20904913689613777No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCCACCGGG5970.20594444674421494No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCG5280.18214182224613984No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC5270.18179685667370396No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATCATTCC5210.17972706323908874No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGC5140.17731230423203764No Hit
GTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGT5120.17662237308716588No Hit
CTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTAAGAAGAAAAGAGAACTCTTCCCGGG5080.17524251079742242No Hit
CCCTTTACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAA5030.17351768293524306No Hit
TTCCAGCACCCGGTGGCGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGCGCGCTTAACAACCAACTTAGAACTG4960.17110292392819196No Hit
TGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCCGGGGAATCT4960.17110292392819196No Hit
GTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCCGCGGATAATC4870.16799823377626913No Hit
TCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTG4860.16765326820383325No Hit
GTATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGC4850.1673083026313974No Hit
CTCTAAGGGTTGGGCGCGTTGGGCCTTGGGCAGATTCCCCGGGAGCAGGTCGGCACTAGCCTCACGGCCGGCGCC4780.16489354362434627No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT4780.16489354362434627No Hit
GTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTG4770.16454857805191042No Hit
CTATGACGTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCCTCTACAAATTACAACTCGGGCTT4690.16178885347242344No Hit
GTCATAGAGGGTGAGAATCCCGTATGTGACCGGCCCGCACCCTTTACGTAGCTCCTTCGACGAGTCGAGTTGTTT4550.15695933545832125No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTT4530.15626940431344952No Hit
CCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGCAAGCCCGAGTTGTAATTTGTAGAG4470.15419961087883427No Hit
GTCACATACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCC4470.15419961087883427No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGG4390.15143988629934732No Hit
ACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACA4150.14316071256088642No Hit
GAGTACGACCTGGCGTGAAAATCATTCCTTCCGGTGGATTTTCAAGGGTCGTCGGGAGCGCACCGGACGGCACAA4130.14247078141601469No Hit
GCCGAAGCCTGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCC4120.1421258158435788No Hit
CTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT3950.136261401112169No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAAC3890.13419160767755378No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATCATTCCTTCCGGTGGATTTTCAAGGGTCGT3860.13315671096024617No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCGCGTTGGGCC3790.13074195195319507No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3770.13005202080832332No Hit
GGTCGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCCGGTGGCGGACGCCCTTGGCAGGCTTCGGCCGTCCGG3680.1269473306564005No Hit
CCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAAAGGGTGCGGGCCGGTCACATAC3620.12487753722178527No Hit
GCTCTAAGGGTTGGGCGCGTTGGGCCTTGGGCAGATTCCCCGGGAGCAGGTCGGCACTAGCCTCACGGCCGGCGC3560.12280774378717005No Hit
ATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGAC3520.12142788149742656No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC3520.12142788149742656No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGCGCGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC3520.12142788149742656No Hit
GTGCCGACCTGCTCCCGGGGAATCTGCCCAAGGCCCAACGCGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT3500.12073795035255483No Hit
TATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCG3490.12039298478011894No Hit
GGTTAGTCGATCCTAAGCCATAGGGAAGTTCCGTTTCAAAGTGCGCGCTACGCGCCGTTTGGCGAAAGGGAAGCC3430.11832319134550372No Hit
TAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGCAAGCCCGAGTTGTAATTTGTAGAGGAT3390.11694332905576024No Hit
CTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCG3380.11659836348332436No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCA3320.11452857004870913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.1114238798967863No Hit
CTACCAGCTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTAAGAAGAAAAGAGAACTCT3200.11038898317947869No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTA3180.10969905203460696No Hit
CTTCTTAACAGCTCATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATAGCTGGTAGAGCGGCACACCTT3170.10935408646217107No Hit
ACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTG3100.10693932745511998No Hit
CACTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCA3070.10590443073781237No Hit
AAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT3070.10590443073781237No Hit
AGCGGCAACAGCTCAAATTTGAAATCTGGCGCAAGCCCGAGTTGTAATTTGTAGAGGATGCTTCTGGGTAGCGAC3050.10521449959294063No Hit
ATGACGTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCCTCTACAAATTACAACTCGGGCTTGC3020.10417960287563302No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATCATTCCTTCCG3010.10383463730319713No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGC3010.10383463730319713No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATC3010.10383463730319713No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA2950.10176484386858191No Hit
ACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGA2940.10141987829614604No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG2940.10141987829614604No Hit
GTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCCTCTACAAATTACAACTCGGGCTTGCGCCAG2920.1007299471512743No Hit
GGATTATCCGCGGCAACGCAACTGAAGGTGGAGACGTCGGCGGGGGCCCCGGGAAGAGTTCTCTTTTCTTCTTAA2920.1007299471512743No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAG2900.10004001600640257No Hit
GTGATGAGCTGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCC2900.10004001600640257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)