FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289884
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT21640.7465054987512246No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCGCGTTG10960.3780822673897145No Hit
ACCCAGAAGCATCCTCTACAAATTACAACTCGGGCTTGCGCCAGATTTCAAATTTGAGCTGTTGCCGCTTCACTC10890.3756675083826634No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACG10240.35324474617433177No Hit
GTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGG9570.33013205282112845No Hit
ATCCTAAGCCATAGGGAAGTTCCGTTTCAAAGTGCGCGCTACGCGCCGTTTGGCGAAAGGGAAGCCGGTTAACAT9070.3128837741993349No Hit
GCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGCAAGCCCGAGTTGTAATTTGTAGA9020.3111589463371556No Hit
GTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTA8320.2870113562666446No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCCTCTACAAATTACAACTCGGGC8050.27769728581087605No Hit
CTTCTGGGTAGCGACCGATCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGTGACCGGCC7500.25872417932690317No Hit
CCTTTGTGCCGTCCGGTGCGCTCCCGACGACCCTTGAAAATCCACCGGAAGGAATGATTTTCACGCCAGGTCGTA7490.2583792137544673No Hit
ACGTAAAGGGTGCGGGCCGGTCACATACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGATCGG7130.24596045314677595No Hit
CCCTTTACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAA6770.23354169253908458No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAAAGGGTGCGGGCCGGTCACATACGGG6750.23285176139421285No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA6360.21939810406921392No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT6340.21870817292434214No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCCGGGGAATCTGCCCAAGGCCCAACGCGCCCAA6230.21491355162754758No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAA6110.2107739647583171No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCCACCGGG6070.20939410246857362No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAAAGGGTGCGGGCCGGTCACATACGGGATTCTCACCCTCT5980.2062894123166508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5910.20387465330959972No Hit
GCACAAAGGTGTGCCGCTCTACCAGCTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTA5810.200424997585241No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCC5580.19249078941921596No Hit
GTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGT5470.1886961681224214No Hit
CTCTAAGGGTTGGGCGCGTTGGGCCTTGGGCAGATTCCCCGGGAGCAGGTCGGCACTAGCCTCACGGCCGGCGCC5290.18248678781857572No Hit
CTTCTTAACAGCTCATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATAGCTGGTAGAGCGGCACACCTT5250.18110692552883223No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCG5070.17489754522498654No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT5060.17455257965255067No Hit
GTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCCGCGGATAATC5050.17420761408011481No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGC5030.17351768293524306No Hit
GTCATAGAGGGTGAGAATCCCGTATGTGACCGGCCCGCACCCTTTACGTAGCTCCTTCGACGAGTCGAGTTGTTT5030.17351768293524306No Hit
CTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTAAGAAGAAAAGAGAACTCTTCCCGGG4980.17179285507306372No Hit
TCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTG4950.17075795835575608No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTT4940.17041299278332023No Hit
TTCCAGCACCCGGTGGCGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGCGCGCTTAACAACCAACTTAGAACTG4760.16420361247947454No Hit
TGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCCGGGGAATCT4750.16385864690703866No Hit
CTATGACGTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCCTCTACAAATTACAACTCGGGCTT4620.15937409446537235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.15902912889293647No Hit
GTATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGC4460.15385464530639842No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCGCGTTGGGCC4440.15316471416152666No Hit
GTCACATACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCC4400.1517848518717832No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA4190.1445405748506299No Hit
CCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGCAAGCCCGAGTTGTAATTTGTAGAG3950.136261401112169No Hit
ACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACA3900.13453657324998966No Hit
CTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT3890.13419160767755378No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATCATTCCTTCCGGTGG3860.13315671096024617No Hit
CCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAAAGGGTGCGGGCCGGTCACATAC3850.1328117453878103No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAAC3720.12832719294614398No Hit
GTTTAATAGCTGGTAGAGCGGCACACCTTTGTGCCGTCCGGTGCGCTCCCGACGACCCTTGAAAATCCACCGGAA3680.1269473306564005No Hit
GCTCTAAGGGTTGGGCGCGTTGGGCCTTGGGCAGATTCCCCGGGAGCAGGTCGGCACTAGCCTCACGGCCGGCGC3670.12660236508396464No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGG3630.12522250279422112No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3570.12315270935960591No Hit
ATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGAC3560.12280774378717005No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCA3510.1210829159249907No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3500.12073795035255483No Hit
CTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCG3480.12004801920768307No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGCGCGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC3420.11797822577306785No Hit
ATGACGTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCCTCTACAAATTACAACTCGGGCTTGC3340.11521850119358089No Hit
TAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGCAAGCCCGAGTTGTAATTTGTAGAGGAT3330.114873535621145No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA3320.11452857004870913No Hit
AGCGGCAACAGCTCAAATTTGAAATCTGGCGCAAGCCCGAGTTGTAATTTGTAGAGGATGCTTCTGGGTAGCGAC3290.11349367333140152No Hit
ACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGA3280.11314870775896566No Hit
GTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTG3270.11280374218652978No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGAC3200.11038898317947869No Hit
TATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCG3160.10900912088973519No Hit
GCCGAAGCCTGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCC3150.10866415531729932No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCG3110.10728429302755585No Hit
CTACCAGCTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTAAGAAGAAAAGAGAACTCT3110.10728429302755585No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAG3100.10693932745511998No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG3090.1065943618826841No Hit
AAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT3030.10452456844806889No Hit
GCCATAGGGAAGTTCCGTTTCAAAGTGCGCGCTACGCGCCGTTTGGCGAAAGGGAAGCCGGTTAACATTCCGGCA3010.10383463730319713No Hit
GTCCGGAGCTAGGGTTTAATAGCTGGTAGAGCGGCACACCTTTGTGCCGTCCGGTGCGCTCCCGACGACCCTTGA2970.10245477501345365No Hit
GTCCTGTTCCAAGGAACTTAGATCGGTCGCTACCCAGAAGCATCCTCTACAAATTACAACTCGGGCTTGCGCCAG2960.10210980944101779No Hit
GGTCGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCCGGTGGCGGACGCCCTTGGCAGGCTTCGGCCGTCCGG2950.10176484386858191No Hit
AAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCGCGTTGGGCCTTGGGCAGATTCCCCGGG2920.1007299471512743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)