FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185139
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG17170.9274112963773165Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG12350.6670663663517681Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT8320.449392078384349Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCAGGTGCTGGAAGG6900.3726929496216357No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6800.3672916025256699Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6400.345686214141807Clontech Universal Primer Mix Long (96% over 26bp)
CTTGTGCGTCGCCCGGCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTGGATTCTCCACGGCAACGTAA6020.32516109517713715No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGTCGCCCGGCGAAAGGGAAGCCGGTTAACA5790.312737996856416No Hit
GTGCTGGAAGGCGCCGGCCCGTAAAGGCTAGTGCCGACCTGCTCCCGGTGCTTCCACCCAAGGCCCAACGTACCC5570.3008550332452914No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG5450.2943734167301325Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTCACGCCAGGTCGTA5410.2922128778917462No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5310.2868115307957805Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTCACGCC5300.2862713960861839No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA5190.2803299142806216No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA5160.2787095101518319No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG5110.276008836603849No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5000.2700673547982867Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT4720.25494358292958264No Hit
TGCCAAGGGCGTCCACAACCAGGTGCTGGAAGGCGCCGGCCCGTAAAGGCTAGTGCCGACCTGCTCCCGGTGCTT4420.23873954164168543No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACA4150.22415590448257797No Hit
GTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCC4060.2192946920962088No Hit
GGCTAGTGCCGACCTGCTCCCGGTGCTTCCACCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCC4030.21767428796741908No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCAGG3950.21335321029064652No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGCCG3810.20579132435629446No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3780.20417092022750474No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA3750.20255051609871502No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3730.20147024667952185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCACGGCAACGTAACTGAAAGCGAAGACGACGGCGGGGGCCCTGGGAAGAGTTCTCTTTTCTTCTTAACGGCC3660.1976893037123459No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT3590.19390836074516984Clontech Universal Primer Mix Long (96% over 26bp)
ACCCATACCTCGCCGCTACAGTAGATTCGACGCTGTAGCGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTTG3590.19390836074516984No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3560.19228795661638012Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3540.19120768719718698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTACTTGTGCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT3530.1906675524875904No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC3460.1868866095204144Clontech Universal Primer Mix Long (96% over 26bp)
GTAGAGTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTC3370.18202539713404522No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3360.18148526242444868No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC3340.18040499300525553No Hit
GAGTACGACCTGGCGTGAAAACTATTCCTTCCAGCGAATTTTCAAGGGACGTCGGGGGCGCACCGGATCCCACAA3330.17986485829565893No Hit
GCCGAAGCCTGCCAAGGGCGTCCACAACCAGGTGCTGGAAGGCGCCGGCCCGTAAAGGCTAGTGCCGACCTGCTC3270.17662405003807952No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGG3250.17554378061888637No Hit
GTTTTAAAGTGCGCACTTGTGCGTCGCCCGGCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTGGATTC3220.17392337649009662No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA3210.17338324178050005No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCG3160.1706825682325172No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGC3150.1701424335229206No Hit
CCACAAAGGTGTGGGACTCTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTA3150.1701424335229206No Hit
ACGCACAAGTGCGCACTTTAAAACGGAGTTTCCCTATGGCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCA3130.16906216410372746No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACC3080.1663614905557446No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT3000.16204041287897203No Hit
GGTGTGGGACTCTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAA2940.1587996046213926No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2840.15339825752542685Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA2810.1517778533966371No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG2790.15069758397744396No Hit
GTAGCGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTTGGGGGTGACCCCGGGTAGAACGGCCTCTAGTGCAG2770.14961731455825084No Hit
GTGCCGACCTGCTCCCGGTGCTTCCACCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT2750.1485370451390577No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT2680.14475610217188167No Hit
GGTTAGTCGATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGTCGCCCGGCGAAAGGGAAGC2500.13503367739914335No Hit
GCCGTGGAGAATCCACATCCAGGTGCCGGAATGTTAACCGGCTTCCCTTTCGCCGGGCGACGCACAAGTGCGCAC2460.13287313856075705No Hit
GTAAAGGCTAGTGCCGACCTGCTCCCGGTGCTTCCACCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTT2450.13233300385116048No Hit
GCCTTGGGGGTGACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC2440.1317928691415639No Hit
GTGTGGGACTCTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAAA2410.13017246501277419No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2370.1280119261743879Clontech Universal Primer Mix Long (96% over 26bp)
GGACTCTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGA2360.12747179146479132No Hit
GAGCTAGGGTTTAATGGCCGGTAGAGTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATT2360.12747179146479132No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA2350.12693165675519474No Hit
ACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACT2350.12693165675519474No Hit
GCCATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGTCGCCCGGCGAAAGGGAAGCCGGTTAACATTCCGGC2350.12693165675519474No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2340.12639152204559817Clontech Universal Primer Mix Long (96% over 26bp)
GGCCGGTAGAGTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAG2290.1236908484976153No Hit
GTCCGGAGCTAGGGTTTAATGGCCGGTAGAGTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGA2290.1236908484976153No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGTGGAAGCACCGGGAGCAGGTCGGCACTAGCCTTTACGGGCCGGC2190.11828950140164957No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC2160.11666909727285986No Hit
CTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGAACTCT2140.11558882785366671No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC2070.1118078848864907No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA2070.1118078848864907No Hit
GCACTAGAGGCCGTTCTACCCGGGGTCACCCCCAAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGCTACAG2070.1118078848864907No Hit
GTGTTACCCATACCTCGCCGCTACAGTAGATTCGACGCTGTAGCGAGTAGGCAGGCGTGGAGGTCAGTGACGAAG2050.11072761546729755No Hit
CTTTAAAACGGAGTTTCCCTATGGCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCC2050.11072761546729755No Hit
CCCCCAAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGCTACAGCGTCGAATCTACTGTAGCGGCGAGGTAT2040.11018748075770096No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2030.1096473460481044Clontech SMART CDS Primer II A (96% over 26bp)
CCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGAACTCTTCCCAGGGCCCCCGC1960.10586640308092839No Hit
GTCCACAACCAGGTGCTGGAAGGCGCCGGCCCGTAAAGGCTAGTGCCGACCTGCTCCCGGTGCTTCCACCCAAGG1960.10586640308092839No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGTGGAAGCACCGGGAGCAGGTCGGCACTAGCCTTTACGGGCCGGCG1950.10532626837133181No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA1950.10532626837133181No Hit
GCGCTCAAGCGTGTTACCCATACCTCGCCGCTACAGTAGATTCGACGCTGTAGCGAGTAGGCAGGCGTGGAGGTC1940.10478613366173524No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT1930.10424599895213867No Hit
TTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTG1920.10370586424254208No Hit
GCCCTGGGAAGAGTTCTCTTTTCTTCTTAACGGCCCGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAAT1860.10046505598496265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)