FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299325
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG16780.560594671343857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG14340.479077925331997Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.34110081015618476No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9640.3220579637517748Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTA8070.26960661488348786No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6340.21180990562098054Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6220.20780088532531532Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.19878058966006848No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5880.1964419944875971Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5780.1931011442412094Clontech Universal Primer Mix Long (96% over 26bp)
AAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC5450.18207633842812998No Hit
GTCGGCGGACTGCTCGAGCTGCTCTCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACCGGGAACG5030.1680447673933016No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4950.16537208719619143Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG4940.16503800217155265No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGTGGCAGCTCGAGCAGTCCGCCGACAG4710.15735404660486094No Hit
GCCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCTCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCG4670.15601770650630586No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4580.15301094128455692Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCGCCCGCGCCCGGTCGTACTCATAAC4420.14766558089033657No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAATTAAA4250.14198613547147748No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAA4080.1363066900526184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.13263175478159192No Hit
GTACATGGGCAGAAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG3950.13196358473231437No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3940.1316294997076756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCGGTCCG3830.12795456443664913No Hit
GACCGGGAACGGCCCCCTCGGGGGCCTTCCCCGGGCGTCGAACAGCCGACTCAGAACTGGTACGGACAAGGGGAA3760.12561596926417773No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT3720.12427962916562266Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3700.12361145911634511Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCGGTCCGTCCCCCGGCCGGCACG3680.12294328906706756No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3630.12127286394387371Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA3580.11960243882067986No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAAAAGCGCCGGTAAGGTG3560.11893426877140233No Hit
GCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT3400.113588908377182No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA3380.11292073832790445No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGCGG3230.10790946295832289No Hit
GTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCGGTCCGTCCCCC3170.10590495281049027No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTC3160.10557086778585151No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAAAGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACG3040.10156184749018625No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCC3000.10022550739163118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)