FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293992
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG66752.2704699447604018Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG57641.9605975672807423Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT32241.0966284796865222Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20860.7095431168195052Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18480.6285885330213067Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA17650.6003564722849601Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16440.5591988897657079Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.5374295899208141No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC14510.4935508449209502Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT14120.48028517782796815Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG12930.4398078859288689Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC12670.4309641078668807Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11270.3833437644561757Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTAATAAGGGCGCACGGTGAATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACGCCGATATGCTTTGG10750.36565620833219953No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9130.31055266809981225Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC8800.2993278728672889Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.2860622057743068No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG7770.264292905929413Clontech Universal Primer Mix Long (96% over 25bp)
CTACAGAGGTGTAGTGCTCTGCCGGCCGTTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGAGAGACCGTTA6980.23742142643337233No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT6720.22857764837138425Clontech SMART CDS Primer II A (96% over 26bp)
GTGAATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACGCCGATATGCTTTGGGGAGCTGTAAGTGAGCT6700.22789735775123132No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6500.22109445154970203Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.20646820321641407No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC5920.2013660235652671Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5520.1877602111622085Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTAAGGTGTGCGAGAGAACTCTCGTTAAGGAACTCGGCAAAATGACCCCGTAACTTCGGGAGAAGGGGTGCT5430.1846989033715203No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5420.18435875806144386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5260.17891643310022043Clontech Universal Primer Mix Long (96% over 26bp)
CCTTAGGATTCTCTCCTCGACTACCTGTGTCGGTTTGCGGTACGGGCAGTTGGTTTCTAACTAGAAGCTTTTCTT4920.16735149255762063No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4860.16531062069716185Clontech Universal Primer Mix Long (96% over 26bp)
CCTCTGTAGTGTCCGGTGCGCTCTTGACGATCCTTGAAAATCCACTGGAAGGAATCAGATTTGTGCCAGGTCGTA4800.16326974883670303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.1510245176739503No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4270.1452420474026504Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA4230.14388146616234457Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAGCACCGGGCAGGCGTCAGCCCCTATACGTCATCTTACGATTTTGCAGAGACCTGTGTTTTTGATAAACAG4200.14286103023211516No Hit
ACCTATACCTCGCCGTTGGGGTTGAAATGATGCCCCAACGAGTAGGCAGGCGTGGGGGTTTGTGACGAAGCCTTG4160.1415004489918093No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT4040.13741870527089173Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA3980.13537783341043294Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCT3780.12857492720890365No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG3740.12721434596859776Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTAAGCCATAGGGTAGTTCCGTTAGAAAGCGCGCAATCTTGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATA3730.12687420065852134No Hit
GTACATGGGTTAAGTTAATAAGGGCGCACGGTGAATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACGC3580.12177202100737436No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT3580.12177202100737436Clontech Universal Primer Mix Long (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3550.12075158507714495Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3490.11871071321668616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTACTGACCTATGTATTCAGTCAGCAGTAACATCCTATCAAAGATGCTGGGTTCCCCCATTCGGAAATCTTTGGA3250.11054722577485102No Hit
ACCCAGGGTCACCCCCAAGGCTTCGTCACAAACCCCCACGCCTGCCTACTCGTTGGGGCATCATTTCAACCCCAA3200.10884649922446869No Hit
GGCCTATTCACTGCGGCTGACCAATTGGTCAGCACCCCTTCTCCCGAAGTTACGGGGTCATTTTGCCGAGTTCCT3140.1068056273640099No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT3120.10612533674385696No Hit
GTATCAACGCAGAGTACATGGGTTAAGTTAATAAGGGCGCACGGTGAATGCCTTGGCACTAGGAGCCGATGAAGG3120.10612533674385696No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA3110.10578519143378051Clontech SMART CDS Primer II A (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3050.10374431957332172Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT3020.10272388364309232Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT2960.10068301178263354Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGGCCCAGGCGACTGTTTATCAAAAACACAGGTCTCTGCAAAATCGTAAGATGACGTATAGGGGCTGACGC2950.10034286647255708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)