FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481522
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG81331.6890194009827175Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG71801.491105286985849Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCCTTAGGAGGAGCATACCTCCCACTTTGCGCAGCATT42250.8774261612138179No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT38060.790410407001134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG28740.5968574644564527Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC25540.5304015185183647Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA24830.5156566055133515Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG23580.4896972516312858Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAGGGATACCCGCTAAACTTAAGCATATCAGTCAGCGGAGGAAAAGAAAGCAACTGCGATTGCCCTAGTAAC22570.4687220936945768No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC20950.43507877106341974Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT19140.39748962664218873Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG17790.3694535244495578Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.3673767761389926No Hit
ACCTCGGATCGGGTAGGGATACCCGCTAAACTTAAGCATATCAGTCAGCGGAGGAAAAGAAAGCAACTGCGATTG17200.3572007094172229No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG16820.34930906583707494Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC16450.34162509708798355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCAAACTACGCGACTCTTCGAAGGAGCGACACACAGGCTTGGCGGCCGACCGTACGGGGCTGTCACCCTCTA16320.3389253242842487No Hit
GTATTTAGCCTTAGGAGGAGCATACCTCCCACTTTGCGCAGCATTCCCAAACTACGCGACTCTTCGAAGGAGCGA13500.2803610219263087No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13240.274961476318839Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12920.26831588172503024Clontech Universal Primer Mix Long (96% over 26bp)
GGTAGGGATACCCGCTAAACTTAAGCATATCAGTCAGCGGAGGAAAAGAAAGCAACTGCGATTGCCCTAGTAACG12860.26706983273869106No Hit
GCCTTAGGAGGAGCATACCTCCCACTTTGCGCAGCATTCCCAAACTACGCGACTCTTCGAAGGAGCGACACACAG12210.25357096872001694No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG10160.21099762835342933Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10080.20933622970497712Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCTGGCCGGTATTTAGCCTTAGGAGGAGCATACCTCCCACTTTGCGCAGCATTCCCAAACTACGCGACTCTT10020.208090180718638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.20580575757701622No Hit
CTTGTGGGGTCCGAGTTGTAATCTGCAGAGGAAGCGTCTGGCGCGGCGCCGGCCTAGTTCCCTGGAACGGGACGC9830.20414435892856403No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC9620.199783187476377Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT9490.19708341467264218Clontech SMART CDS Primer II A (96% over 26bp)
CCCTAGTAACGGCGAGTGAAGCGGCAGCAGCCCAGATTTAAAATCTGGCCCTCTTGTGGGGTCCGAGTTGTAATC8930.18545362413347677No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7980.16572451518310688Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG7170.14890285386752838Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG7130.14807215454330228Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTTGTGGGGTCCGAGTTGTAATCTGCAGAGGAAGCGTCTGGCGCGGCGCCGGCCTAGTTCCCTGGAACGGGAC7070.14682610555696313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.1457877314016805No Hit
GTACGGGGCTGTCACCCTCTACGGCGTCCCGTTCCAGGGAACTAGGCCGGCGCCGCGCCAGACGCTTCCTCTGCA6950.14433400758428483No Hit
GTATCAACGCAGAGTACATGGGGTCGACCTCGGATCGGGTAGGGATACCCGCTAAACTTAAGCATATCAGTCAGC6860.1424649341047761No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6690.13893446197681517Clontech Universal Primer Mix Long (96% over 26bp)
CTATCGGTCTCTGGCCGGTATTTAGCCTTAGGAGGAGCATACCTCCCACTTTGCGCAGCATTCCCAAACTACGCG6490.13478096535568468No Hit
GCCCTAGTAACGGCGAGTGAAGCGGCAGCAGCCCAGATTTAAAATCTGGCCCTCTTGTGGGGTCCGAGTTGTAAT6350.13187351772089334No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG6260.1300044442413846Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA5860.12169745099912362Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5640.11712860471588006Clontech Universal Primer Mix Long (96% over 26bp)
CCGTAGAGGGTGACAGCCCCGTACGGTCGGCCGCCAAGCCTGTGTGTCGCTCCTTCGAAGAGTCGCGTAGTTTGG5630.11692092988482355No Hit
ATCGGTCTCTGGCCGGTATTTAGCCTTAGGAGGAGCATACCTCCCACTTTGCGCAGCATTCCCAAACTACGCGAC5600.11629790539165397No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5590.11609023056059745Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT5520.11463650674320175Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5450.11318278292580608Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCCCACTTTGCGCAGCATTCCCAAACTACGCGACTCTTCGAAGGAGCGACACACAGGCTTGGCGGCCGACCG5230.10861393664256254No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT5160.10716021282516687Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACACAGGCTTGGCGGCCGACCGTACGGGGCTGTCACCCTCTACGGCGTCCCGTTCCAGGGAACTAGGCCGGCG4970.10321439103509289No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT4940.10259136654192332Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGTCGACCTCGGATCGGGTAGGGATACCCGCTAAACTTAAGCATATCAGTCAGCGGAGGAAAAGAAA4890.1015529923866407No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT4840.10051461823135806Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)