FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002150033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635464
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG46790.7363123638789923Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG44420.699016781438445Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG32760.515528810444022No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT29220.45982148477333096No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA29130.4584051968325507No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG25860.40694673498420053No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT22570.3551735424823436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGTCAGTTCTAAGCTAATCGTTAAGTGTATATTGACGTTATA21560.33927964448025383No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT19940.31378646154620876No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG19240.30277088867347324No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCC17460.27475986051137435No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA17260.27161255397630707No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA17040.26815051678773305No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACAAATAGTCAACGCACATAAACGCCTTCTATTTGCTCTGTTTTA16920.2662621328666927No Hit
GTTCTAAGCTAATCGTTAAGTGTATATTGACGTTATACCCGAAAGCATAACAAGTTTAAGAAATCCACAAAACAA16540.2602822504500648No Hit
TTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAACTG16400.25807913587551773No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGG16300.2565054826079841No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGC16020.2520992534588899No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC15990.2516271574786298No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCG15230.23966739264537407No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA15140.23825110470459382Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG15060.2369921820905669Clontech Universal Primer Mix Long (96% over 26bp)
GTGTATATTGACGTTATACCCGAAAGCATAACAAGTTTAAGAAATCCACAAAACAAGTTAAACACTAGTCCAAGT14840.23353014490199286No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14800.2329006835949794Clontech Universal Primer Mix Long (96% over 26bp)
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTG14270.22456032127705108No Hit
GCTATATATGGCCCTTGAAAATATGAGGGATCCCTAATAATGTATTCCATAGCCGTACCTTATTCCGCATCAGGT14080.22157038006873717No Hit
ACCTTATTCCGCATCAGGTTTTCAAGGTGAATAGCCTCTGGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAA13540.2130726524240555No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTG13310.2094532499087281No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12910.20315863683859353Clontech Universal Primer Mix Long (96% over 26bp)
TTCCTACACTATGAGAATACGATTTTACGAAGAATTTCGGTTCTTTGTAAAAGGGACGGTAACGTTATATGAACT12900.2030012715118402No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC12220.19230042929261137Clontech Universal Primer Mix Long (96% over 26bp)
TTCCATAGCCGTACCTTATTCCGCATCAGGTTTTCAAGGTGAATAGCCTCTGGTCGATAGAATAATGTAGGTAAG12080.19009731471806426No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAG12040.18946785341105082No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT11880.186950008182997No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT11870.1867926428562436No Hit
ATATTGACGTTATACCCGAAAGCATAACAAGTTTAAGAAATCCACAAAACAAGTTAAACACTAGTCCAAGTTCAG11710.1842747976281898No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT11690.18396006697468306No Hit
CCCTAATAATGTATTCCATAGCCGTACCTTATTCCGCATCAGGTTTTCAAGGTGAATAGCCTCTGGTCGATAGAA11600.1825437790339028No Hit
GTATTTGAATGTGGGTCAGTCGGTCCTAAGGAAATAGCGCATGCTATTTTCATTATGTGTGTTTTCATATTTATA11570.18207168305364269No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCC10670.16790880364583988No Hit
GTATCAACGCAGAGTACATGGGATTTTCACGCGAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGC10550.16602041972479953No Hit
GTACATGGGCAGAAATCACAAATAGTCAACGCACATAAACGCCTTCTATTTGCTCTGTTTTAATTAGACAGTCGG10550.16602041972479953No Hit
ACGCAGAGTACATGGGCAGAAATCACAAATAGTCAACGCACATAAACGCCTTCTATTTGCTCTGTTTTAATTAGA10530.1657056890712928No Hit
CTATTTGCTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGTCAGTTCTAAGCTAATCGTTAAGTGTATA10400.16365993982349905No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTT10390.16350257449674568No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10310.16224365188271878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10280.16177155590245867Clontech Universal Primer Mix Long (96% over 26bp)
GGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGG9950.15657850011959765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.15421802021829717No Hit
ATATATGGCCCTTGAAAATATGAGGGATCCCTAATAATGTATTCCATAGCCGTACCTTATTCCGCATCAGGTTTT9660.1520149056437501No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT9650.15185754031699672No Hit
ATATATGACGTATATGAGTTCATATAACGTTACCGTCCCTTTTACAAAGAACCGAAATTCTTCGTAAAATCGTAT9500.14949706041569624No Hit
GTCATATATAGTTCAGAATAGAGATCTCTTCTCTATTTAAGATATTTTGTCCATGGAATGAGATCATCTCGAGAT9490.14933969508894288No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACAAATAGTCAACGCACATAAACGCCTTCTATTTGCTCTGTTTTAA9420.14823813780166933No Hit
GTACATGGGATTTTCACGCGAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAG9130.14367454332582177No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG9130.14367454332582177No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG9110.14335981267231504No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA9040.1422582553850415No Hit
TAATTAGACAGTCGGATTCCCCGAGTCCGTGTCAGTTCTAAGCTAATCGTTAAGTGTATATTGACGTTATACCCG8890.139897775483741No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG8750.1376946609091939Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA8610.1354915463346468No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA8590.1351768156811401No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC8440.13281633577983962Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG8410.13234423979957952No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA8380.13187214381931944No Hit
GTTAAACACTAGTCCAAGTTCAGCCGAAGCATCCCTCAAACATGATCTAACTCATCAAGTTTCATCCAAAAACCC8280.13029849055178577No Hit
ATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC8270.1301411252250324No Hit
GCCGTACCTTATTCCGCATCAGGTTTTCAAGGTGAATAGCCTCTGGTCGATAGAATAATGTAGGTAAGGGAAGTC8230.12951166391801897No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACC8170.1285674719574988No Hit
CCCCGAGTCCGTGTCAGTTCTAAGCTAATCGTTAAGTGTATATTGACGTTATACCCGAAAGCATAACAAGTTTAA8170.1285674719574988No Hit
CATAAACGCCTTCTATTTGCTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGTCAGTTCTAAGCTAATC8130.12793801065048532No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGAC7690.12101393627333727No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC7620.11991237898606373No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC7600.119597648332557Clontech Universal Primer Mix Long (96% over 26bp)
ATCACAAATAGTCAACGCACATAAACGCCTTCTATTTGCTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCG7600.119597648332557No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7550.11881082169879018Clontech Universal Primer Mix Long (96% over 26bp)
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGG7520.11833872571853007No Hit
GAATAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGAATCGAGTA7500.11802399506502334No Hit
GAATGAGATCATCTCGAGATATGGATAAAGGTATTGAAAAGCATAACGGTTTTTGTTATGTTCTGATTGCTATAT7480.11770926441151662No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGC7400.1164503417974897No Hit
GGATAAAGGTATTGAAAAGCATAACGGTTTTTGTTATGTTCTGATTGCTATATATGGCCCTTGAAAATATGAGGG7300.11487668852995607No Hit
CTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGCC7240.11393249656943588No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA7210.11346040058917578No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG7200.11330303526242243No Hit
TGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCT7170.11283093928216231No Hit
CTAATAATGTATTCCATAGCCGTACCTTATTCCGCATCAGGTTTTCAAGGTGAATAGCCTCTGGTCGATAGAATA7060.11109992068787532No Hit
ACATTATTCTATCGACCAGAGGCTATTCACCTTGAAAACCTGATGCGGAATAAGGTACGGCTATGGAATACATTA7030.11062782470761523No Hit
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCCT6680.10512003827124747No Hit
ATATATAGCAATCAGAACATAACAAAAACCGTTATGCTTTTCAATACCTTTATCCATATCTCGAGATGATCTCAT6470.10181536640942682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)