FastQCFastQC Report
Wed 8 May 2024
EGAF00002150046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894967
Total Bases67.1 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGGAA146281.6344736733309722No Hit
GTCCGTCCACGGGCCGGAGCCCGCGACCGACGACCGACACCTCGCAGCAA59940.6697453649128963No Hit
GTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTGGACGGTCCGTCCA57830.6461690766251716No Hit
GTATCGGGCTTGGTCGGGAAGCGTAGACGTGCTAAGTGCCGGGCCTGGGC50350.5625905759653708No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCG45570.5091807854367815No Hit
CTCTATAAGCGTTCGAGTTCCCTGGAATCCTTTAGCAGGGAGATAGGGTT43630.48750400852768877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42030.4696262543758597No Hit
GCGCGGGACCGAGCAGAGAATCGGCCGCCCGAACCCCCGGGGGGGAACTG37640.42057416642177864No Hit
TTCCAATACGGGCCCTCACCGAGTGCTCGACGGGGCAACCCAAAGAGACC37350.4173338234817596No Hit
GCCCGATACAGCCCCGATCCTCAGAGCCAATCCTTGTTCCGAAGTTACGG35150.3927519115229947No Hit
CTCACCGAGTGCTCGACGGGGCAACCCAAAGAGACCCGGAGACGCCGTCG34680.3875003212408949No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAA34640.38705337738709916No Hit
ACCCAAAGAGACCCGGAGACGCCGTCGGGAGATCCGGAGAGAGTTTTCTT33310.3721924942483913No Hit
GTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAGAGGGCTACGTGGA31500.3519682848641347No Hit
GCGTAGACGTGCTAAGTGCCGGGCCTGGGCGAGATGAATCCGTCAGTTCC31230.3489514138510135No Hit
GTGCCGGGCCTGGGCGAGATGAATCCGTCAGTTCCCCCCCGGGGGTTCGG30990.3462697507282391No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCC29930.3344257386026524No Hit
TAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTTCTGAGTTGATCGT29640.3311853956626334No Hit
GGGCTGTATCGGGCTTGGTCGGGAAGCGTAGACGTGCTAAGTGCCGGGCC29030.32436950189224856No Hit
GTTCCAATACGGGCCCTCACCGAGTGCTCGACGGGGCAACCCAAAGAGAC28320.3164362484873744No Hit
ATACAGCCCCGATCCTCAGAGCCAATCCTTGTTCCGAAGTTACGGATCAA28200.31509541692598725No Hit
GTACATGGGGGCACCCACCGGGCGAAAGGGAATCCGGTTCCTATTCCGGA27550.30783257930180663No Hit
GAAGTGGACGGTCCGTCCACGGGCCGGAGCCCGCGACCGACGACCGACAC27010.3017988372755644No Hit
GGCCGGAGCCCGCGACCGACGACCGACACCTCGCAGCAAGGAAGATCCGC26670.2979998145183007No Hit
GAATAATGTAGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAAC26600.2972176627741581No Hit
TCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCA25170.2812394200009609No Hit
TGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTGGACGGTCC25080.2802337963299205No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGA24790.2769934533899015No Hit
CGTCTACGCTTCCCGACCAAGCCCGATACAGCCCCGATCCTCAGAGCCAA24450.2731944306326378No Hit
GTCCCGCGCCTTGGCCTCACGCGGATCTTCCTTGCTGCGAGGTGTCGGTC24200.27040103154641454No Hit
AAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTA24170.27006582365606774No Hit
GGCCCGGCACTTAGCACGTCTACGCTTCCCGACCAAGCCCGATACAGCCC23090.25799833960358315No Hit
GTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTTCTGAGTTGA22990.25688097996909387No Hit
GAGATAGGGTTTGGAACGCGAAGAGCACCGCAGTTGCAGCGGTGTCCGGA22880.25565188437115555No Hit
GGCCCTCACCGAGTGCTCGACGGGGCAACCCAAAGAGACCCGGAGACGCC22470.2510707098697494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22020.24604259151454747No Hit
GTACATGGGGCACCCACCGGGCGAAAGGGAATCCGGTTCCTATTCCGGAA21160.23643329865793936No Hit
CCCAAAGAGACCCGGAGACGCCGTCGGGAGATCCGGAGAGAGTTTTCTTT20890.23341642764481818No Hit
GTCCACGGGCCGGAGCCCGCGACCGACGACCGACACCTCGCAGCAAGGAA20850.23296948379102245No Hit
GGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCCG20370.22760615754547375No Hit
CTATAAGCGTTCGAGTTCCCTGGAATCCTTTAGCAGGGAGATAGGGTTTG19680.21989637606749748No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGA19550.21844380854266135No Hit
ACATTATTCTATCGACTAGAGGCTCTTAACCTTGGAGACCTGCTGCGGAT19250.2150917296391934No Hit
GCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGT19110.21352742615090833No Hit
GTGCTCGACGGGGCAACCCAAAGAGACCCGGAGACGCCGTCGGGAGATCC19090.2133039542240105No Hit
ATGTAGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAGGA18960.2118513866991744No Hit
GGTTAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAA18160.20291250962325985No Hit
GTAGCGGAACCGTTCCAATACGGGCCCTCACCGAGTGCTCGACGGGGCAA17760.1984430710853026No Hit
CCGTGGAAGTGGACGGTCCGTCCACGGGCCGGAGCCCGCGACCGACGACC17670.1974374474142622No Hit
GCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTTCTGA17290.1931914808032028No Hit
GTGTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAGAGGGCTACGTG17060.19062155364387737No Hit
GTATCAACGCAGAGTACATGGGGGCACCCACCGGGCGAAAGGGAATCCGG17000.1899511378631838No Hit
GTCGGGAAGCGTAGACGTGCTAAGTGCCGGGCCTGGGCGAGATGAATCCG16760.18726947474040942No Hit
TTGCAGCGGTGTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAGAGG16710.18671079492316478No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGC16610.18559343528867545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.1844760756541861No Hit
GTTGAATGGCGACCGTGGAAGTGGACGGTCCGTCCACGGGCCGGAGCCCG16380.18302350812935003No Hit
AGGAAGATCCGCGTGAGGCCAAGGCGCGGGACCGAGCAGAGAATCGGCCG16370.1829117721659011No Hit
CTTCCCGACCAAGCCCGATACAGCCCCGATCCTCAGAGCCAATCCTTGTT16060.17944795729898422No Hit
CTCCAAGGTTAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGT15420.1722968556382526No Hit
CAGCAAGGAAGATCCGCGTGAGGCCAAGGCGCGGGACCGAGCAGAGAATC14990.16749220920994853No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTTAAGAGCCTCTAGTCGATAGAAT14880.16626311361201027No Hit
GACCGACACCTCGCAGCAAGGAAGATCCGCGTGAGGCCAAGGCGCGGGAC14740.16469881012372523No Hit
GGTTTGGAACGCGAAGAGCACCGCAGTTGCAGCGGTGTCCGGATCTTCCC14640.16358145048923592No Hit
GATCGTACCCATATCCGCAGCAGGTCTCCAAGGTTAAGAGCCTCTAGTCG14610.16324624259888912No Hit
GGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAGGATTGGC14600.16313450663544019No Hit
GTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTGGAC14220.1588885400243808No Hit
ATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAAT14100.15754770846299362No Hit
CCTCAGAGCCAATCCTTGTTCCGAAGTTACGGATCAAGTTTGCCGACTTC13680.15285479799813848No Hit
GTACCCATATCCGCAGCAGGTCTCCAAGGTTAAGAGCCTCTAGTCGATAG13490.15073181469260877No Hit
TACCTACATTATTCTATCGACTAGAGGCTCTTAACCTTGGAGACCTGCTG13410.14983792698501733No Hit
GCTCTGAGGATCGGGGCTGTATCGGGCTTGGTCGGGAAGCGTAGACGTGC13380.14950271909467053No Hit
GGGCTACGTGGAAAGTTCGCACCGGATCGTACCCATATCCGCAGCAGGTC12760.14257508936083677No Hit
AGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAGGATTGG12720.14212814550704103No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAGTCTCGTGC12490.13955821834771562No Hit
ATACGGGCCCTCACCGAGTGCTCGACGGGGCAACCCAAAGAGACCCGGAG12330.1377704429325327No Hit
TCCTTTAGCAGGGAGATAGGGTTTGGAACGCGAAGAGCACCGCAGTTGCA12320.13765870696908378No Hit
GCTAAGTGCCGGGCCTGGGCGAGATGAATCCGTCAGTTCCCCCCCGGGGG11930.13330100439457546No Hit
GTATCAACGCAGAGTACATGGGGCACCCACCGGGCGAAAGGGAATCCGGT11700.13073107723525002No Hit
CCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTTGATCC11660.1302841333814543No Hit
ACCGACGACCGACACCTCGCAGCAAGGAAGATCCGCGTGAGGCCAAGGCG11600.1296137176007607No Hit
ATCCGCAGCAGGTCTCCAAGGTTAAGAGCCTCTAGTCGATAGAATAATGT11380.1271555264048842No Hit
ATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGG11240.12559122291659916No Hit
GTACATGGGACAGAGGGAATCTCGTTAATCCATTCATGCGCGTCACTAAT11030.1232447676841716No Hit
GTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCG11030.1232447676841716No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGCTTGAAT10940.1222391440131312No Hit
GACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCCT10790.12056310456139724No Hit
CAGTTGCAGCGGTGTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAG10440.11665234584068464No Hit
GTTGCAGCGGTGTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAGAG10210.11408241868135921No Hit
GAGCAGAGAATCGGCCGCCCGAACCCCCGGGGGGGAACTGACGGATTCAT10170.11363547482756348No Hit
CTCTTAACCTTGGAGACCTGCTGCGGATATGGGTACGATCCGGTGCGAAC10110.11296505904686989No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTCTTAACCTTGGAGACCT10080.11262985115652309No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGC10060.11240637922962522No Hit
TATCAACGCAGAGTACATGGGGGCACCCACCGGGCGAAAGGGAATCCGGT9950.11117728363168697No Hit
TCCCTTACCTACATTATTCTATCGACTAGAGGCTCTTAACCTTGGAGACC9870.11028339592409553No Hit
CGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCT9860.1101716599606466No Hit
CCCTCACCGAGTGCTCGACGGGGCAACCCAAAGAGACCCGGAGACGCCGT9750.10894256436270834No Hit
GTCCACGTACCGGCGACGCATCTTTCAAATGTCTGACTTATCAACTGTCG9740.10883082839925941No Hit
ATCCGGGAGAGGGCTACGTGGAAAGTTCGCACCGGATCGTACCCATATCC9670.1080486766551169No Hit
GGACCGAGCAGAGAATCGGCCGCCCGAACCCCCGGGGGGGAACTGACGGA9400.10503180564199574No Hit
ATCGTACCCATATCCGCAGCAGGTCTCCAAGGTTAAGAGCCTCTAGTCGA9360.10458486178820002No Hit
GCCCCGATCCTCAGAGCCAATCCTTGTTCCGAAGTTACGGATCAAGTTTG9350.10447312582475107No Hit
CCTTTAGCAGGGAGATAGGGTTTGGAACGCGAAGAGCACCGCAGTTGCAG9340.10436138986130215No Hit
CCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCC9310.10402618197095535No Hit
ACGCAGAGTACATGGGGGCACCCACCGGGCGAAAGGGAATCCGGTTCCTA9280.10369097408060857No Hit
GCACTTAGCACGTCTACGCTTCCCGACCAAGCCCGATACAGCCCCGATCC9270.10357923811715962No Hit
GTTAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAA9180.10257361444611923No Hit
GCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTTGATC8970.10022715921369169No Hit
GGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTGGA8960.10011542325024274No Hit
CCCTGGAATCCTTTAGCAGGGAGATAGGGTTTGGAACGCGAAGAGCACCG8950.10000368728679382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)