FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002150047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894967
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTGGACGGTCCGTCCACGGGCCGGA73710.8236057865820752No Hit
GTATCGGGCTTGGTCGGGAAGCGTAGACGTGCTAAGTGCCGGGCCTGGGCGAGATGAATCCGTCAGTTCCCCCCC39940.446273438015033No Hit
GTCCGTCCACGGGCCGGAGCCCGCGACCGACGACCGACACCTCGCAGCAAGGAAGATCCGCGTGAGGCCAAGGCG38290.42783700404595926No Hit
GTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTGGACGGTCCGTCCACGGGCCGGAGCCCGCGACCGACGAC32750.36593528029525113No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTA27520.30749737141145983No Hit
GCGCGGGACCGAGCAGAGAATCGGCCGCCCGAACCCCCGGGGGGGAACTGACGGATTCATCTCGCCCAGGCCCGG26090.2915191286382626No Hit
GCCCGATACAGCCCCGATCCTCAGAGCCAATCCTTGTTCCGAAGTTACGGATCAAGTTTGCCGACTTCCCTTACC26050.29107218478446695No Hit
CTCTATAAGCGTTCGAGTTCCCTGGAATCCTTTAGCAGGGAGATAGGGTTTGGAACGCGAAGAGCACCGCAGTTG24980.27911643669543124No Hit
GTGCCGGGCCTGGGCGAGATGAATCCGTCAGTTCCCCCCCGGGGGTTCGGGCGGCCGATTCTCTGCTCGGTCCCG24890.27811081302439083No Hit
GGGCTGTATCGGGCTTGGTCGGGAAGCGTAGACGTGCTAAGTGCCGGGCCTGGGCGAGATGAATCCGTCAGTTCC23250.25978611501876603No Hit
GCGTAGACGTGCTAAGTGCCGGGCCTGGGCGAGATGAATCCGTCAGTTCCCCCCCGGGGGTTCGGGCGGCCGATT23110.258221811530481No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGG21590.24123794508624338No Hit
ATACAGCCCCGATCCTCAGAGCCAATCCTTGTTCCGAAGTTACGGATCAAGTTTGCCGACTTCCCTTACCTACAT20950.23408684342551178No Hit
GTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAGAGGGCTACGTGGAAAGTTCGCACCGGATCGTACCCATA20410.2280531013992695No Hit
GTCCCGCGCCTTGGCCTCACGCGGATCTTCCTTGCTGCGAGGTGTCGGTCGTCGGTCGCGGGCTCCGGCCCGTGG19600.21900248835990602No Hit
GAATAATGTAGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAGGATTGGCTCTGAGGATCGGGGC18960.2118513866991744No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGA18770.2097284033936447No Hit
CGTCTACGCTTCCCGACCAAGCCCGATACAGCCCCGATCCTCAGAGCCAATCCTTGTTCCGAAGTTACGGATCAA18730.20928145953984897No Hit
TTCCAATACGGGCCCTCACCGAGTGCTCGACGGGGCAACCCAAAGAGACCCGGAGACGCCGTCGGGAGATCCGGA18520.2069350043074214No Hit
CTCACCGAGTGCTCGACGGGGCAACCCAAAGAGACCCGGAGACGCCGTCGGGAGATCCGGAGAGAGTTTTCTTTT18200.20335945347705556No Hit
GGCCCGGCACTTAGCACGTCTACGCTTCCCGACCAAGCCCGATACAGCCCCGATCCTCAGAGCCAATCCTTGTTC17000.1899511378631838No Hit
TAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTGGACG16860.18838683437489873No Hit
GGCCGGAGCCCGCGACCGACGACCGACACCTCGCAGCAAGGAAGATCCGCGTGAGGCCAAGGCGCGGGACCGAGC16350.18268830023900323No Hit
ACCCAAAGAGACCCGGAGACGCCGTCGGGAGATCCGGAGAGAGTTTTCTTTTCTCTATAAGCGTTCGAGTTCCCT16330.18246482831210536No Hit
GAAGTGGACGGTCCGTCCACGGGCCGGAGCCCGCGACCGACGACCGACACCTCGCAGCAAGGAAGATCCGCGTGA15940.17810712573759704No Hit
TCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCG15720.17564893454172054No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAA15490.17307900738239512No Hit
GAGATAGGGTTTGGAACGCGAAGAGCACCGCAGTTGCAGCGGTGTCCGGATCTTCCCCTCGGACCTTGAAAATCC15210.16995040040582501No Hit
AAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTC15170.16950345655202928No Hit
ACATTATTCTATCGACTAGAGGCTCTTAACCTTGGAGACCTGCTGCGGATATGGGTACGATCCGGTGCGAACTTT14870.16615137764856136No Hit
ATGTAGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAGGATTGGCTCTGAGGATCGGGGCTGTAT14810.16548096186786773No Hit
GTTCCAATACGGGCCCTCACCGAGTGCTCGACGGGGCAACCCAAAGAGACCCGGAGACGCCGTCGGGAGATCCGG14300.15978242773197224No Hit
TGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTGGACGGTCCGTCCACGGGCCGGAGCCCGCGACCG14040.15687729268230002No Hit
GGTTAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAG13940.1557599330478107No Hit
GTACATGGGGGCACCCACCGGGCGAAAGGGAATCCGGTTCCTATTCCGGAACCCGGTAGCGGAACCGTTCCAATA13410.14983792698501733No Hit
GTCCACGGGCCGGAGCCCGCGACCGACGACCGACACCTCGCAGCAAGGAAGATCCGCGTGAGGCCAAGGCGCGGG12910.14425112881257074No Hit
GTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTG12450.13911127449391988No Hit
GGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAA12290.13732349907873698No Hit
CTATAAGCGTTCGAGTTCCCTGGAATCCTTTAGCAGGGAGATAGGGTTTGGAACGCGAAGAGCACCGCAGTTGCA11920.1331892684311265No Hit
CTTCCCGACCAAGCCCGATACAGCCCCGATCCTCAGAGCCAATCCTTGTTCCGAAGTTACGGATCAAGTTTGCCG11600.1296137176007607No Hit
GGCCCTCACCGAGTGCTCGACGGGGCAACCCAAAGAGACCCGGAGACGCCGTCGGGAGATCCGGAGAGAGTTTTC11560.12916677374696497No Hit
GTCGGGAAGCGTAGACGTGCTAAGTGCCGGGCCTGGGCGAGATGAATCCGTCAGTTCCCCCCCGGGGGTTCGGGC11340.12670858255108847No Hit
GTGCTCGACGGGGCAACCCAAAGAGACCCGGAGACGCCGTCGGGAGATCCGGAGAGAGTTTTCTTTTCTCTATAA11330.12659684658763956No Hit
CCCAAAGAGACCCGGAGACGCCGTCGGGAGATCCGGAGAGAGTTTTCTTTTCTCTATAAGCGTTCGAGTTCCCTG11190.1250325430993545No Hit
GGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAGGATTGGCTCTGAGGATCGGGGCTGTATCGGGC11170.12480907117245663No Hit
CCTCAGAGCCAATCCTTGTTCCGAAGTTACGGATCAAGTTTGCCGACTTCCCTTACCTACATTATTCTATCGACT11140.12447386328210985No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGGAAGTGGACGGTCCGTCCAC10950.12235087997658015No Hit
GTGTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAGAGGGCTACGTGGAAAGTTCGCACCGGATCGTACCCA10870.12145699226898869No Hit
CTCCAAGGTTAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGG10780.1204513685979483No Hit
GCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGTGG10760.12022789667105044No Hit
AGGAAGATCCGCGTGAGGCCAAGGCGCGGGACCGAGCAGAGAATCGGCCGCCCGAACCCCCGGGGGGGAACTGAC10700.11955748089035685No Hit
GTACCCATATCCGCAGCAGGTCTCCAAGGTTAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGC10670.11922227300001005No Hit
GATCGTACCCATATCCGCAGCAGGTCTCCAAGGTTAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGT10630.11877532914621432No Hit
GCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTT10610.11855185721931646No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTT10500.11732276162137822No Hit
CCGTGGAAGTGGACGGTCCGTCCACGGGCCGGAGCCCGCGACCGACGACCGACACCTCGCAGCAAGGAAGATCCG10240.114417626571706No Hit
GTACATGGGGCACCCACCGGGCGAAAGGGAATCCGGTTCCTATTCCGGAACCCGGTAGCGGAACCGTTCCAATAC10040.11218290730272737No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTTAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAA10030.11207117133927842No Hit
GCTAAGTGCCGGGCCTGGGCGAGATGAATCCGTCAGTTCCCCCCCGGGGGTTCGGGCGGCCGATTCTCTGCTCGG9990.1116242274854827No Hit
TACCTACATTATTCTATCGACTAGAGGCTCTTAACCTTGGAGACCTGCTGCGGATATGGGTACGATCCGGTGCGA9950.11117728363168697No Hit
CAGCAAGGAAGATCCGCGTGAGGCCAAGGCGCGGGACCGAGCAGAGAATCGGCCGCCCGAACCCCCGGGGGGGAA9900.11061860381444233No Hit
GCTCTGAGGATCGGGGCTGTATCGGGCTTGGTCGGGAAGCGTAGACGTGCTAAGTGCCGGGCCTGGGCGAGATGA9860.1101716599606466No Hit
TTGCAGCGGTGTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAGAGGGCTACGTGGAAAGTTCGCACCGGAT9790.10938950821650407No Hit
GGTTTGGAACGCGAAGAGCACCGCAGTTGCAGCGGTGTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAGAG9560.10681958105717865No Hit
GACCGACACCTCGCAGCAAGGAAGATCCGCGTGAGGCCAAGGCGCGGGACCGAGCAGAGAATCGGCCGCCCGAAC9550.10670784509372973No Hit
GTTGAATGGCGACCGTGGAAGTGGACGGTCCGTCCACGGGCCGGAGCCCGCGACCGACGACCGACACCTCGCAGC9250.10335576619026177No Hit
GGGCTACGTGGAAAGTTCGCACCGGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTTAAGAGCCTCTAGTCGA9240.10324403022681285No Hit
AGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAGGATTGGCTCTGAGGATCGGGGCTGTATCGGG9200.1027970863730171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)