FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002150052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751963
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86081.1447371745684296No Hit
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC62330.8288971664829253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47320.6292862813728867No Hit
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA46620.6199773127135244No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG37870.5036152044714967Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG35630.4738265047615374Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG31360.41704179593942786No Hit
TATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTG27490.36557649777981094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26720.3553366322545125No Hit
GTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCT25130.334191974871104No Hit
GTATAGGATACACGTTGATGTCTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTT24070.3200955366154984No Hit
GTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCC23170.3081268626248898No Hit
CTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCA22120.2941634096358464No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC20960.2787371187146176No Hit
GTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGG20370.2708909879874409No Hit
ATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACT20150.2679653121230699No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT19790.26317784252682647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTATAGCTGCTCACAGAAAGCAGGTGGTTATCACATAAGATCCGCTTAGGCTTTTTCTGCTGTAGCTTCAGTT19410.2581244023974584No Hit
GTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTC19080.2537358886009019No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18950.2520070801355918No Hit
TACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAA17440.23192630488468183No Hit
GTCTATCACTACTACTTCATTTATAGCTGCTCACAGAAAGCAGGTGGTTATCACATAAGATCCGCTTAGGCTTTT17400.23139436381843254No Hit
GCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAA16850.2240801741575051No Hit
GTATCAACGCAGAGTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGT16620.22102151302657178No Hit
GTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCG15910.2115795591006472No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTA15510.20626014843815454No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAA15160.20160566410847344No Hit
CAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGC14860.1976161061116039No Hit
ATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTA14590.1940255039144213No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14380.19123281331661265No Hit
ATATTAAATAGATACCTATCAGCTGTTGAGATGCTTGATACCTATTAGTCGGGTGAGTGGTTCGCCATGTATCCT14160.18830713745224165No Hit
GGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATT13810.18365265312256057No Hit
GGATACACGTTGATGTCTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGC13490.17939712459256638No Hit
GTATACGTTTGTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAG13170.17514159606257224No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAA12960.17234890546476356No Hit
GTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAA12670.16849233273445635No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12660.16835934746789405Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA12120.16117814307352887Clontech Universal Primer Mix Long (96% over 26bp)
GGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGG12080.1606462020072796No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.15851843774228253No Hit
GTGTAGTTTCACCTGATAGTTTGTCTACCTCCTTGTGGGGTGACTTTATCTGCTTAATAGGACAATTTGTGTTTA11920.15851843774228253No Hit
ATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGT11910.15838545247572022No Hit
GCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAG11440.1521351449472913No Hit
ATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCCTT11200.14894349854979566No Hit
TACAAAGACCGAAAGGGAATCCGGTTACAATTCCGGAACCTGTTGAGTATACGTTTGTATAAGGTATCTTGTATA11120.14787961641729713No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11120.14787961641729713Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10890.14482095528636382Clontech Universal Primer Mix Long (96% over 26bp)
GTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTC10640.14149632362230588No Hit
GTTGTACTCACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTG10440.13883661829105953No Hit
GTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAAGCAGATAAAGTCACCCCACAAGGAGGTAGA10360.137772736158561No Hit
GTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCCCGTACCTTTCGGTATAGGATACACGTTGAT10330.13737378035887404No Hit
GTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAAC10210.13577795716012622No Hit
GGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAG10080.1340491486948161No Hit
GTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGG9970.1325863107626306No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9430.12540510636826546Clontech Universal Primer Mix Long (96% over 26bp)
TTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGAC9320.12394226843607996No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAA9310.12380928316951766No Hit
CCTATCAGCTGTTGAGATGCTTGATACCTATTAGTCGGGTGAGTGGTTCGCCATGTATCCTTGCTGGTAGGTGTA9290.12354331263639301No Hit
GTTATACACTTTGGTTGGGCTGGCAACGGCCCTTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAAC9240.12287838630358143No Hit
CTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCA9240.12287838630358143No Hit
ATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTT9110.12114957783827131No Hit
CTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCT9110.12114957783827131No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGA9100.12101659257170898No Hit
TAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGA9080.12075062203858433No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.12061763677202203No Hit
CACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATA9010.11981972517264812No Hit
CTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCT8940.1188888283067119No Hit
TATCAACGCAGAGTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGTT8850.11769196090765104No Hit
CTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGA8820.11729300510796409No Hit
GTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTT8650.11503225557640469No Hit
GTACATGGGATATACAAAGACCGAAAGGGAATCCGGTTACAATTCCGGAACCTGTTGAGTATACGTTTGTATAAG8630.11476628504328006No Hit
CGTTTGTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGT8490.11290449131140762No Hit
CATAAGATCCGCTTAGGCTTTTTCTGCTGTAGCTTCAGTTGCGACTGAACCATTGTCAACAGGTGCTGGTGGAGA8240.10957985964734968No Hit
GATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGAT8030.106787169049541No Hit
CCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATT8030.106787169049541No Hit
CTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTC8000.10638821324985405No Hit
TTTATATACAATTTAAATTGTCGTTTTTTTATATCGTCTATCACTACTACTTCATTTATAGCTGCTCACAGAAAG7990.10625522798329173No Hit
CTACTACTTCATTTATAGCTGCTCACAGAAAGCAGGTGGTTATCACATAAGATCCGCTTAGGCTTTTTCTGCTGT7970.1059892574501671No Hit
CTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCC7920.10532433111735552No Hit
GCTTGATACCTATTAGTCGGGTGAGTGGTTCGCCATGTATCCTTGCTGGTAGGTGTAGTTTCACCTGATAGTTTG7750.10306358158579611No Hit
CCATGTATCCTTGCTGGTAGGTGTAGTTTCACCTGATAGTTTGTCTACCTCCTTGTGGGGTGACTTTATCTGCTT7720.10266462578610916No Hit
CTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGC7720.10266462578610916No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7710.10253164051954684Clontech Universal Primer Mix Long (96% over 26bp)
AGTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGT7640.1016007436536106No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACTTCCCTTACCTACATTGATCTATCGACT7620.10133477312048599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)