FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002150053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751963
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC49580.6593409516159705No Hit
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA36190.48127367968902723No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG27380.3641136598476255Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG26600.3537408090557647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG24660.3279416673426751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22510.2993498350317768No Hit
GTATAGGATACACGTTGATGTCTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTT21970.29216863063741166No Hit
TATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTG21600.28724817577460593No Hit
GTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCT21320.283524588310861No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC19030.25307096226809034No Hit
GTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCC18820.2502782716702816No Hit
CTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCA17740.23591586288155136No Hit
ATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACT17120.22767077635468766No Hit
GTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGG17030.22647390895562683No Hit
ATTTATAGCTGCTCACAGAAAGCAGGTGGTTATCACATAAGATCCGCTTAGGCTTTTTCTGCTGTAGCTTCAGTT16670.22168643935938337No Hit
GTCTATCACTACTACTTCATTTATAGCTGCTCACAGAAAGCAGGTGGTTATCACATAAGATCCGCTTAGGCTTTT15230.2025365609744096No Hit
TACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAA14990.19934491457691403No Hit
GTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTC14950.19881297351066474No Hit
GCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAA14670.19508938604691986No Hit
GTATCAACGCAGAGTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGT13320.177136375061007No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13310.17700338979444466Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAA13210.1756735371288215No Hit
CAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGC13180.17527458132913454No Hit
GTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCG13010.17301383179757515No Hit
ATATTAAATAGATACCTATCAGCTGTTGAGATGCTTGATACCTATTAGTCGGGTGAGTGGTTCGCCATGTATCCT12940.17208293493163893No Hit
GGATACACGTTGATGTCTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGC11950.1589173935419695No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTA11700.15559276187791155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.15333201234635213No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAA11230.14934245434948262No Hit
GGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGG11120.14787961641729713No Hit
GTATACGTTTGTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAG10840.14415602895355226No Hit
ATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTA10810.14375707315386527No Hit
GGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATT10800.14362408788730296No Hit
ATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCCTT10470.13923557409074647No Hit
GTGTAGTTTCACCTGATAGTTTGTCTACCTCCTTGTGGGGTGACTTTATCTGCTTAATAGGACAATTTGTGTTTA10470.13923557409074647No Hit
GTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAA10380.13803870669168564No Hit
GCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAG9690.12886272329888573No Hit
GGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAG9590.12753287063326255No Hit
ATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGT9530.12673495903388865No Hit
GTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCCCGTACCTTTCGGTATAGGATACACGTTGAT9400.1250061505685785No Hit
GTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAAC9180.12208047470420752No Hit
GTTATACACTTTGGTTGGGCTGGCAACGGCCCTTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAAC8760.11649509350859018No Hit
GTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAAGCAGATAAAGTCACCCCACAAGGAGGTAGA8750.11636210824202786No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8540.11356941764421921Clontech Universal Primer Mix Long (96% over 26bp)
GTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTC8490.11290449131140762No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8430.11210657971203371Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGG8330.11077672704641053No Hit
TACAAAGACCGAAAGGGAATCCGGTTACAATTCCGGAACCTGTTGAGTATACGTTTGTATAAGGTATCTTGTATA8230.10944687438078735No Hit
CTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCA8110.10785105118203955No Hit
CCTATCAGCTGTTGAGATGCTTGATACCTATTAGTCGGGTGAGTGGTTCGCCATGTATCCTTGCTGGTAGGTGTA8070.10731911011579026No Hit
CTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCT7890.10492537531766855No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7810.10386149318517002Clontech Universal Primer Mix Long (96% over 26bp)
GTTGTACTCACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTG7770.10332955211892075No Hit
TATCAACGCAGAGTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGTT7740.10293059631923379No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAA7730.10279761105267149No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7620.10133477312048599Clontech Universal Primer Mix Long (96% over 26bp)
CTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGA7550.10040387625454975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)