FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129585
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34842.688582783501177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21171.633676737276691No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15491.1953544005864876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.7979318593973068No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA9230.7122737971215805TruSeq Adapter, Index 5 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.3426322491029054No Hit
ATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4030.3109927846587182TruSeq Adapter, Index 5 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3730.28784195701663TruSeq Adapter, Index 5 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.2816684029787398No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.2646911293745418No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.2068140602693213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT1650.12732955203148513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1490.11498244395570474No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA1490.11498244395570474TruSeq Adapter, Index 5 (96% over 28bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.1134390554462322No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.10649380715360574No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA1350.10417872438939692TruSeq Adapter, Index 5 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA1310.10109194737045182RNA PCR Primer, Index 5 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)