FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154785
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46132.9802629453758436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30571.9749975772846207No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA17461.1280162806473495TruSeq Adapter, Index 5 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15931.0291694931679427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.8224311141260457No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.5401040152469555No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.3811738863584973No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5850.37794359918596765No Hit
ATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4590.29654036243822074TruSeq Adapter, Index 5 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.28103498401007854No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2980.1925251154827664TruSeq Adapter, Index 5 (96% over 28bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.1544077268469167No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.14859320993636332No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.12985754433569144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT1760.11370610847304324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1740.1124139936040314No Hit
ACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1620.10466130438996027RNA PCR Primer, Index 5 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)