FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660990
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG50200.7594668603155872No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGG42030.6358643852403214No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT33760.5107490279731918No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT30300.45840330413470703No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCA27770.42012738468055494No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGG25900.39183648769270335No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAG24260.3670252197461384No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA24060.36399945536241096No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG23260.35189639782750115No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGTTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC21360.32315163618209053No Hit
GCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCA20400.3086279671401988No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAAT18690.2827576816593292No Hit
TGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCT18520.28018578193316085No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT18440.2789754761796699No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA18390.278219035083738No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTG18370.2779164586453653No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT18120.274134253165706No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC17240.26082088987730523No Hit
GTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCC16390.24796139124646363No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA16240.24569206795866808TruSeq Adapter, Index 5 (96% over 28bp)
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC16180.24478433864354981No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTAC15750.23827894521853582No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA15520.23479931617724925No Hit
GCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCCCAACGTACCCAA15280.23116839891677635No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGG14990.22678104056037154No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG14960.22632717590281246No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCC14750.22315012329989864No Hit
TTTCAGCGCTGGGGTGCAGACACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGTTTAACAACCAACTTAGAACTG14500.21936791782023934No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCG14150.2140728301487163No Hit
GAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGCGCACCAAACGCCGCAA13620.2060545545318386No Hit
GGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCT13320.20151590795624746No Hit
GAAGGGCACTAACCGCAAGGTGGGCGCCTTTCAGCGCTGGGGTGCAGACACCCTTGGCAGGCTTCGGCCGTCCGG13250.20045689042194284No Hit
GGGTACGTTGGGCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCCTTTCAGCGCTG13170.19924658466845185No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT13150.1989440082300791No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC13040.19727983781902902No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGC12960.19606953206553804No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT12870.1947079380928607No Hit
CCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC12850.19440536165448796No Hit
ACTTATACCCCTCCGCTGGGGCAAAATTTACGCCCCAGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTG12270.1856306449416784No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA11790.17836881042073255No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGT11630.17594819891375058No Hit
CGTTGGGCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCCTTTCAGCGCTGGGGTG11470.17352758740676863No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTC11380.1721659934340913No Hit
GGCTTCGGCCGTCCGGCGTACGTTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATT11300.17095568768060032No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA10510.15900391836487693No Hit
GTTGGACACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGA10220.15461656000847215No Hit
GTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT10120.1531036778166084No Hit
GTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCC10100.15280110137823566No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT9900.14977533699450823No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGC9860.14917018411776276No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC9460.14311865535030788No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT9420.14251350247356237No Hit
AACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATT9300.14069804384332593No Hit
GGGTTGGGTACGTTGGGCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCCTTTCAG9270.14024417918576682No Hit
CTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCCCA9200.13918516165146222No Hit
CCGCAAGGTGGGCGCCTTTCAGCGCTGGGGTGCAGACACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGTTTAAC9170.1387312969939031No Hit
GCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCCTTTCAGCGCTGGGGTGCAGACA9150.13842872055553035No Hit
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCCT8480.12829240987004342No Hit
CCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCT8380.1267795276781797No Hit
GCGCAGAAGGGCACTAACCGCAAGGTGGGCGCCTTTCAGCGCTGGGGTGCAGACACCCTTGGCAGGCTTCGGCCG8380.1267795276781797No Hit
GGGGTGCAGACACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGTTTAACAACCAACTTAGAACTGGTACGGACAA8370.12662823945899332No Hit
GGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTGTTGACGCAAT8330.12602308658224784No Hit
GTTAGTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCG8280.125266645486316No Hit
GTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTAC8250.12481278082875688No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA8080.12224088110258854No Hit
CTTCTGCGCCAGAGGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGG8020.12133315178747033No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGC7990.12087928712991118No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGG7940.12012284603397934No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTA7940.12012284603397934No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC7840.11860996384211561No Hit
GTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATC7760.11739965808862463No Hit
CTTCTTGGGGCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAATT7730.11694579343106551No Hit
ACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA7660.11588677589676091No Hit
GGTGCAGACACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGTTTAACAACCAACTTAGAACTGGTACGGACAAGG7510.11361745260896534No Hit
CCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCCCAACGTA7460.11286101151303349No Hit
GTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGCGCACCAAACGC7420.112255858636288No Hit
ACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCT7250.10968395891011967No Hit
GGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCTCCCCAA7230.10938138247174693No Hit
CTCTTAGGATCGATTGACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTC7170.10847365315662869No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG7140.10801978849906957No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAC7120.10771721206069684No Hit
TGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCA7010.10605304164964674No Hit
GGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAATTCCGGCATCTGGA6950.1051453123345285No Hit
CGTCGGGAGCGCACCAAACGCCGCAAAAGTGCAGCGCTCTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGAT6950.1051453123345285No Hit
CTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGG6880.1040862948002239No Hit
GTGCAGACACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGTTTAACAACCAACTTAGAACTGGTACGGACAAGGG6870.10393500658103752No Hit
GCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCTC6810.1030272772659193No Hit
CAGTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAATTTTAACCGGCTTCCCTTTCGAGGGCGAGGCGCA6800.10287598904673294No Hit
GTGAAGGGCATGAGGTTCTTCAGAAGGAAGGCCCGGCCGGACGAGTGCACGCGGTGAGGCGGACCCGCCAGCCAG6800.10287598904673294No Hit
GTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGG6800.10287598904673294No Hit
GCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAACG6780.10257341260836018No Hit
GGCACTAACCGCAAGGTGGGCGCCTTTCAGCGCTGGGGTGCAGACACCCTTGGCAGGCTTCGGCCGTCCGGCGTA6670.10090924219731011No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCGTAAATTTTGCCCCAG6670.10090924219731011No Hit
GCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC6620.10015280110137824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)