FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286050
Sequences flagged as poor quality0
Sequence length75
%GC24

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78032.7278447823807026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47021.6437685719279844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37281.3032686593252927No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA33811.1819611955951757TruSeq Adapter, Index 5 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20660.7222513546582765No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA10490.3667191050515644TruSeq Adapter, Index 5 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA9460.33071141408844607TruSeq Adapter, Index 5 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7940.27757385072539764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT6570.22968012585212377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT6080.21255025345219367No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.18563188253801782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT4310.1506729592728544No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.14542912078307987No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.14123404999126027Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTT3730.1303967837790596No Hit
TATACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA3570.12480335605663347RNA PCR Primer, Index 5 (96% over 28bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.12235623142807202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTT3410.1192099283342073No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.11606362524034258No Hit
ACACATCTCCGAGCCCACGAGACGACGCGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA3290.11501485754238769RNA PCR Primer, Index 5 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT3170.11081978675056808No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.10277923439958049No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2880.10068169900367069No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)