FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280382
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63092.2501444457918125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38311.3663501936643578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25860.9223131299441476No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA23670.8442054054825202TruSeq Adapter, Index 1 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.6048890442325113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.255365893673631No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.24644948677161874No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.23146992317623813No Hit
ATACACATCTCCGAGCCCACGAGACCTGCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6130.21863029723734045TruSeq Adapter, Index 1 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCTGCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5380.19188107653130373TruSeq Adapter, Index 1 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.15443216754285224No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)