FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327362
Sequences flagged as poor quality0
Sequence length20-100
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180712.770877499526517No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59261.810228432133235No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30030.917333105247402No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28170.8605152705567536No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21120.6451573487454255No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14830.45301531637758813No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10010.305777701749134No Hit
ATGGGAAGCAGTAGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA9100.2779797288628491No Hit
ATGGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA7970.24346136692713266No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC7170.21902358856556353No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6820.208332060532377No Hit
ATGGGAAGCAGTGATATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5240.16006744826827793No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4760.14540478125133646No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4460.136240614365748No Hit
ATGGGGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.13532419767718917No Hit
ATGGGAAGCGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.1337968365295911No Hit
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4330.13226947538199302No Hit
ATGGGAAGCAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4290.13104758646391457No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC3930.12005058620120845No Hit
ATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.11363566938129654No Hit
ATGGGAAGCTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.10997000262706116No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT3480.10630433587282581No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.10141678020051197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)