FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289738
Sequences flagged as poor quality0
Sequence length20-100
%GC34

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250811.21979167385707No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40541.3991951349149923No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25140.8676804561362335No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22460.7751830964526573No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19760.6819954579654722No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC16260.5611966673339362No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14600.5039035266344076No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11640.4017422637003085No Hit
ATGGGAAGCAGTGGTATCAACGC11210.38690126942271985No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA9280.3202893648744728No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8460.2919879339265129Clontech Universal Primer Mix Long (96% over 26bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT8430.29095251572109976No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT8160.28163375187238127No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7870.271624709220054No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC7270.2509163451117907No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA6550.22606630818187468No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG5780.1994905742429367No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC5680.19603918022489283No Hit
ATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAA5510.19017181039421824No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5500.18982667099241385No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT5320.18361416175993484No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG5100.1760210949202383Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC4670.16118010064264957No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG4520.15600300961558375No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4460.1539321732047574No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA4250.14668424576686523No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT4220.14564882756145206No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC4190.1446134093560389No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4030.13909117892716868No Hit
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.1352946455073204No Hit
ATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.1332238090964941No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3790.13080783328386333Clontech Universal Primer Mix Long (95% over 21bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3780.13046269388205894No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3760.1297724150784502No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3690.12735643926581947No Hit
ATGGGAAGCAGTGGTATCAAC3690.12735643926581947No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA3570.1232147664441668No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC3530.12183420883694923No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC3500.12079879063153609No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG3440.11872795422070974No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT3410.11769253601529657No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG3320.11458628139905708No Hit
ATGGGGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3300.1138960025954483No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA3240.11182516618462197No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT3210.1107897479792088No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3110.10733835396116492No Hit
ATGGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.10699321455936053No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTT3010.10388695994312101No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG2930.10112584472868591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)